CO2 fixation adjustment mechanism explained by the chemical energy production and consumption balance in photosynthesis

光合作用化学能产生与消耗平衡解释的CO2固定调节机制

• 批准号: 05454049
• 负责人: KUBOTA Fumitake
• 金额: $ 4.29万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454049/
• 关键词: Photosynthesis; Photochemical system; CO2 fixation system; RuBPCarboxylase; Intermittent illumination; Epidermis peeling; カンショ; C_3植物; C_4植物; RuBPカルボキシラーゼ; RuBPオキシゲナーゼ

The aim of this experimental research is to understand, based on the CO2 exchange rate, the mutual adjustment mechanism between photochemical system and CO2 fixation system from the viewpoint of the sink-source balance in chemical energy produced and consumed for photosynthesis, and to obtain fundamental knowledge for increasing the energy utilization efficiency in leaf photosynthesis. Through this study, using leaves of sweet potato (Ipomoea batatas) and mungbean (Vigna radiata), the author has developed new experimental techniques and obtained some interesting results by applying the developed methods. By using the epidermis peeling technique devised here, CO2 exchange rate of the mesophyll can be measured with an assimilation chamber using a leaf without epidermis or stomatal resistance, and from the measurements, the in vivo activity and activation status of the main photosynthetic enzyme (RuBPCase/Oase) are estimated. Also to change chemical energy status in a leaf by intermittent illuminations, the rotating sector of high speed has been devised and improved. Knowledge of the photosynthetic response to intermittent illuminations can become a clue to understand the mutual adjustment mechanism in photosynthesis based on the sink-source balabce of chemical energy. Further detailed study to be made from now on may promise to give more distinctive and correct explanation on the adjustment mechanism for photosynthesis and establish physiological and genetical theory and method to increase the photosynthetic utilization efficiency.

本实验研究的目的是从光合作用产生和消耗化学能的库源平衡的角度，以CO2交换速率为基础，了解光化学系统和CO2固定系统之间的相互调节机制，为提高叶片光合作用的能量利用效率提供基础知识。通过这项研究，利用甘薯（Ipomoea batatas）和绿豆（Vigna radiata）的叶片，作者开发了新的实验技术，并通过应用所开发的方法获得了一些有趣的结果。通过使用表皮剥离技术，叶肉的CO2交换率可以用同化室使用没有表皮或气孔阻力的叶片测量，并从测量中，估计体内活性和主要光合酶（RuBPCase/Oase）的活化状态。此外，为了通过间歇光照改变叶片中的化学能状态，设计并改进了高速旋转扇区。光合作用对间歇光照的响应可以成为理解基于化学能库源平衡的光合作用相互调节机制的线索。今后的深入研究，有望对光合作用的调节机制作出更明确、更正确的解释，为提高光合利用效率建立生理学和遗传学理论和方法。

项目成果

Fumitake Kubota et al.: "Photosynthetic Control Factors in a Single Leaf of Sweet Potato, Ipomoea batatas Lam. 2.Sink-source relationship between photosynthesis and root weight production in single leaf grafts." Japanese Journal of Crop Science. 62-2. 248

Fumitake Kubota 等人：“甘薯单叶光合控制因素，Ipomoea batatas Lam。2.单叶嫁接中光合作用与根重产生之间的库-源关系。”

Fumitake,Kubota: "Stomatal movement and CO2 exchange rate of sweet potato plant (Ipomoea batatas Lam.)in relation to water environments-A comparison between native and improved varieties-." Journal of The Faculty of Agriculture Kyushu Unio.38. 97-110 (199

Fumitake，Kubota：“甘薯植物（Ipomoea batatas Lam.）的气孔运动和二氧化碳交换率与水环境的关系 - 本地品种和改良品种之间的比较 -”。

Fumitake,Kubota: "Photosynthetic control factors in a single leaf of sweet potato, Ipomoea batatas Lam.3.Estimation of in vivo rubisco activity from CO2 exchange rate of a peeled leaf." 日本作物学会紀事. 63. 89-95 (1993)

Fumitake，Kubota：“甘薯单叶光合控制因素，Ipomoea batatas Lam.3。根据去皮叶子的 CO2 交换率估算体内 rubisco 活性。”日本作物科学学会公报 63. 89。 -95 (1993 )

Fumitake Kubota,: "Stomatal movement and CO2 exchange rate of sweet potato plant(Ipomoea batatas Lam.)in relation to water environments." Jomnal of The Faculty of Agicultwre Kyushu Univ.38. 97-110 (1993)

Fumitake Kubota：“甘薯植物（Ipomoea batatas Lam.）的气孔运动和二氧化碳交换率与水环境的关系。”

窪田文武: "Photusyntetic Contnl Foctors in a Single Lesf of Sueet Potota,IpomoeaBatatas Lam,3" 日本作物学会紀事. 63. 89-95 (1994)

Fumitake Kubota：“Sueet Potota、IpomoeaBatatas Lam 的单一 Lesf 中的光合控制因子，3”日本作物科学学会杂志 63. 89-95 (1994)。