权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

FLOWERING OF CULTIVATED PLANTS

栽培植物的开花

基本信息

批准号：
05454050
负责人：
OSAMU Tanaka
金额：
$ 4.42万
依托单位：
KONAN UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454050/
关键词：
Flowering Nitrogen deficiency Lemna Vigna angularis Phaseolus vulgaris Glycine max 花成花成誘導物質リジン

项目摘要

1.We established the culture systems of Vigna angularis, Glycine max and Phaseolus vulgaris, with the removal of the root and the decapitation of the terminal bud from the seedling, for the studies of the mechanism of flowering. In those systems, small size of the cultivated plants permits experiments in a minimal amount of space and the flowering of Vigna angularis and Glycine max can be observed on the 40th day after the start of the culture. Flowering of Phaseolus vulgaris can be obtained even on the 15th day of the culture.2. The addition of casamino acids or peptone to the medium suppressed the flower induction of these plants. Elastational, a protease inhibitor, also suppressed the flowering of these plants. These results suggest that the flower induction of these plants can be induced by nitrogen deficiency, as is the case for Lemna palnts.3. The high-molecular-weight substance, probably protein, that has flower-inducing activity in Lemna paucicostata, 151 was found in these plants. However, it has been not clear whether the substance is involved in the control of flowering of these plants in nature. We found that the substance involve the processes of flower induction of these plants, in nature.4. The products (s) degradated from the protein (s) by protease has a promotive effect on flower induction of Lemna. For understanding the mechanism of flowering at molecular level, we tried to isolate the protein (s) before the degradation by protease. The protein that had been separated by SDS-PAGE was blotted onto PVDF membranes. At present, a pure preparation is subjecting to protein microsequencing.5.We also determined the amino-terminal amino acid sequence of a flower-specific protein in Cuscuta japonica, for the studies of the genetic control of flowering. The amino terminal of this protein was determined to be Ala-Lys-Ile-Lys-Ile-Gly-Ile-Asn-Gly-Phe on a protein sequencer.

1.以赤豆、大豆和菜豆为材料，采用去根、去顶芽的方法，建立了赤豆、大豆和菜豆的离体培养体系，研究了赤豆、大豆和菜豆的开花机理。在这些系统中，栽培植物的小尺寸允许在最小量的空间中进行实验，并且在培养开始后的第40天可以观察到豇豆和大豆的开花。菜豆在培养的第15天就能开花.培养基中添加酪蛋白氨基酸或蛋白胨抑制了这些植物的成花诱导。一种蛋白酶抑制剂，也抑制了这些植物的开花。这些结果表明，这些植物的成花诱导可以被氮素缺乏所诱导，就像浮萍一样.在这些植物中发现了高分子量物质，可能是蛋白质，在浮萍151中具有诱导开花的活性。然而，目前尚不清楚该物质是否参与控制自然界中这些植物的开花。我们发现该物质在自然界中参与了这些植物的成花诱导过程.蛋白酶降解产物对浮萍的成花有促进作用。为了从分子水平上理解开花的机制，我们试图在蛋白酶降解之前分离蛋白质。将通过SDS-PAGE分离的蛋白质印迹到PVDF膜上。目前，纯品正在进行蛋白质微序列测定。5.我们还测定了日本菟丝子花特异性蛋白的氨基端氨基酸序列，为研究开花的遗传控制奠定了基础。经蛋白质序列测定，该蛋白质的氨基末端为Ala-Lys-Ile-Lys-Ile-Gly-Ile-Asn-Gly-Phe。