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Forms of retranslocated nitrogen during leaf senescence and nitrogen deficiency

叶片衰老和缺氮期间氮再转运的形式

基本信息

批准号：
111001152
负责人：
Professor Dr. Nicolaus von Wirén
金额：
--
依托单位：
Abteilung Physiologie und Zellbiologie
依托单位国家：
德国
项目类别：
Research Units
财政年份：
2009
资助国家：
德国
起止时间：
2008-12-31 至 2014-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/111001152?language=en
关键词：
Forms retranslocated nitrogen during leaf

项目摘要

Nitrogen (N) retranslocation from senescing leaves to the grains is one of the limiting factors for an efficient utilization of fertilizer N. Our previous studies showed that urea-N can account for >13% of retranslocated amino acid-N and that the urea transporter DUR3 can increase N retranslocation by retrieval of apoplastic urea upstream of phloem loading. The present Project 5 Forms of retranslocated nitrogen 3 project aims at investigating further the contribution of different N forms and their sense-cence-regulated transporters to N retrieval in senescing leaves and to N retranslocation. In barley, the retranslocation efficiency of 15N-labeled urea and other N forms will be assessed in wild-type and transgenic plants with altered expression of membrane transporters or transcription factors. A regulatory link of these N transporters to upstream transcription factors and to salicylic acid as a putative leaf-inherent signal will be investigated in the model species Arabidopsis. To determine the relevance of leaf-inherent signals for N retranslocation in rapeseed, physiological and molecular analyses will be performed in hydroponics and in the field. Emphasis will be placed on rapeseed hybrid lines that have an outstanding N efficiency under field conditions.Keywords: phloem loading, nitrogen retranslocation, AMT, DUR3, nitrogen starvation, nitrogen efficiency.

从衰老叶片到籽粒的氮素重转运是氮素有效利用的限制因素之一。我们的研究表明，尿素氮可占重转运氨基酸氮的约13%，尿素转运体DUR3可通过韧皮部负荷上游的外质体尿素回收来增加氮素重转运。Project 5 Forms of retranslocated氮素3项目旨在进一步研究不同氮素形态及其感官调控转运体对衰老叶片氮素回收和氮素重转运的贡献。在大麦中，将在膜转运蛋白或转录因子表达改变的野生型和转基因植株中评估15n标记尿素和其他N形式的重易位效率。这些N转运体对上游转录因子和水杨酸作为叶片固有信号的调控联系将在模式物种拟南芥中进行研究。为了确定叶片固有信号与油菜籽氮重转运的相关性，将在水培和田间进行生理和分子分析。重点将放在田间条件下氮效率突出的油菜籽杂交种上。关键词：韧皮部负荷，氮重转运，AMT, DUR3，氮饥饿，氮效率