Patho-physiological study of piloplasmosis in domestic animals
家畜毛虫病的病理生理学研究
基本信息
- 批准号:05454122
- 负责人:
- 金额:$ 4.22万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1993
- 资助国家:日本
- 起止时间:1993 至 1994
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The mechanisms of destruction of nonparasitized erythrocytes and thrombo-cytopenia in dogs infected with Babesia gibsoni were studied. The results were as follows.1. The membrane proteins from erythrocytes of the infected dogs showed a markedly reduced level of band 4.1a compared to that from normal dogs. The concentrations of both cholesterol and phospholipids in the erythrocyte menbranes from the infected dogs were higher than those from normal dogs.2. When B.gibsoni was cultured together with erythrocytes, marked increase of methemoglobin (metHb) and malondialdehyde concentrations of erythrocytes in the cultures were observed together with high multiplication of the parasites, but not at low multiplication of the parasites in the culture. When erythrocytes from the infected dogs were incubated with acetylphenyl-hydrazine, the metHb formation in erythrocytes was much higher at day 3 than at day 0 after the inoculation of the parasites.3. Addition of erythrocytes or the supernatant from B.gibsoni-infected erythrocyte culture to canine platelet-rich plasma showed enhanced platelet aggregation with ADP.These results indicate that nonparasitized erythrocytes of dogs infected with B.gibsoni are sensitive to oxidative stress, and that the factor which promoted canine platelet aggregation may be associated with thrombocytopenia observed in canine babesiosis.
本文研究了吉氏巴氏丝虫感染犬后,非寄生红细胞的破坏和血小板减少的机理。研究结果如下:1.与正常犬相比,感染犬红细胞膜蛋白中4.1a带的水平明显降低。感染犬红细胞膜胆固醇和磷脂含量均高于正常犬.当吉氏B.gibsoni与红细胞一起培养时,培养物中高铁血红蛋白(metHb)和丙二醛浓度显著增加,同时观察到寄生虫的高增殖,但在培养物中寄生虫的低增殖时不观察到。将感染犬的红细胞与乙酰苯肼孵育后,红细胞内metHb的生成量在感染后第3天明显高于第0天.将感染B. gibsoni的红细胞或培养上清加入犬富血小板血浆中,均能增强ADP诱导的血小板聚集,提示感染B.gibsoni的犬非寄生红细胞对氧化应激敏感,促进犬血小板聚集的因子可能与犬巴贝斯虫病血小板减少症有关。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
MURASE,T., IWAI,M.& MAEDE,Y.: "Direct evidence for preferential multiplication of Babesia gibsoni in young erythrocytes" Parasitol Res. 79. 269-271 (1993)
村濑 T.、岩井 M.
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Murase,T.: "Direct evidence for preferential multiplication of Babesia gibsoni in young erythrocytes" Parasitol Res. 79. 269-271 (1993)
Murase,T.:“吉氏巴贝虫在年轻红细胞中优先增殖的直接证据”Parasitol Res。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Murase,T.: "Oxidative damage and enhanced erythrophagocytosis in canine erythrocytes infected with Babesia gibsoni" Parasitol Res. (発表予定).
Murase, T.:“感染吉氏巴贝虫的犬红细胞中的氧化损伤和增强的噬红细胞作用”Parasitol Res。
- DOI:
- 发表时间:
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- 影响因子:0
- 作者:
- 通讯作者:
Murase,T.: "Mechanism of thrombocytopenia in dogs infected with Babesia gibsoni" Am J Vet Res. (発表予定).
Murase, T.:“感染吉布氏巴贝虫的狗的血小板减少机制”Am J Vet Res。
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- 影响因子:0
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MAEDE Yoshimitsu其他文献
MAEDE Yoshimitsu的其他文献
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{{ truncateString('MAEDE Yoshimitsu', 18)}}的其他基金
THE ROLE OF MACROPHAGES ON THE PATHOGENESIS OF BABESIOSIS
巨噬细胞在巴贝斯虫病发病机制中的作用
- 批准号:
11460142 - 财政年份:1999
- 资助金额:
$ 4.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Pathogenesisi of anemia induced by babesia in domestic animals
巴贝斯虫所致家畜贫血的发病机制
- 批准号:
08456156 - 财政年份:1996
- 资助金额:
$ 4.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Culture of piroplasms using some components of reticulocytes for the purpose of cell-free culture of the parasite.
使用网织红细胞的某些成分培养梨质体,以实现寄生虫的无细胞培养。
- 批准号:
06556049 - 财政年份:1994
- 资助金额:
$ 4.22万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Clinico-pathological study on the mechanism of anemia in piroplasmosis of domestic animals
家畜梨形虫病贫血机制的临床病理学研究
- 批准号:
03454110 - 财政年份:1991
- 资助金额:
$ 4.22万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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10661759 - 财政年份:2022
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Identifying the Target of a Potent and Selective Inhibitor of Babesia Blood Stages.
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10527782 - 财政年份:2022
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10042448 - 财政年份:2020
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