Patho-physiological study of piloplasmosis in domestic animals

本文研究了吉氏巴氏丝虫感染犬后，非寄生红细胞的破坏和血小板减少的机理。研究结果如下：1.与正常犬相比，感染犬红细胞膜蛋白中4.1a带的水平明显降低。感染犬红细胞膜胆固醇和磷脂含量均高于正常犬.当吉氏B.gibsoni与红细胞一起培养时，培养物中高铁血红蛋白（metHb）和丙二醛浓度显著增加，同时观察到寄生虫的高增殖，但在培养物中寄生虫的低增殖时不观察到。将感染犬的红细胞与乙酰苯肼孵育后，红细胞内metHb的生成量在感染后第3天明显高于第0天.将感染B. gibsoni的红细胞或培养上清加入犬富血小板血浆中，均能增强ADP诱导的血小板聚集，提示感染B.gibsoni的犬非寄生红细胞对氧化应激敏感，促进犬血小板聚集的因子可能与犬巴贝斯虫病血小板减少症有关。

The mechanisms of destruction of nonparasitized erythrocytes and thrombo-cytopenia in dogs infected with Babesia gibsoni were studied. The results were as follows.1. The membrane proteins from erythrocytes of the infected dogs showed a markedly reduced level of band 4.1a compared to that from normal dogs. The concentrations of both cholesterol and phospholipids in the erythrocyte menbranes from the infected dogs were higher than those from normal dogs.2. When B.gibsoni was cultured together with erythrocytes, marked increase of methemoglobin (metHb) and malondialdehyde concentrations of erythrocytes in the cultures were observed together with high multiplication of the parasites, but not at low multiplication of the parasites in the culture. When erythrocytes from the infected dogs were incubated with acetylphenyl-hydrazine, the metHb formation in erythrocytes was much higher at day 3 than at day 0 after the inoculation of the parasites.3. Addition of erythrocytes or the supernatant from B.gibsoni-infected erythrocyte culture to canine platelet-rich plasma showed enhanced platelet aggregation with ADP.These results indicate that nonparasitized erythrocytes of dogs infected with B.gibsoni are sensitive to oxidative stress, and that the factor which promoted canine platelet aggregation may be associated with thrombocytopenia observed in canine babesiosis.