Culture of piroplasms using some components of reticulocytes for the purpose of cell-free culture of the parasite.

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To clarify the factors which enhance the multiplication of Babesia gibsoni in canine reticulocytes in vitro.1. Babesia parasites were cultured together with canine erythrocytes with hereditary high concentration of glutamate, or cells with low glutamate.2. Babesia parasites were cultured in the medium containing several materials which present in reticulocytes.(Results)1. The highest multiplication of the parasites was observed in the erythrocytes containing high glutamate.2.(1) A high multiplication of the parasites was observed when the parasites were cultured with glucose or glutamate.(2) The multiplication of the parasites was increased when the parasites were cultured together with resealed cells containing sediments of hemolysate of reticulocytes.(Conclusion)These results indicate that both glutamate and glucose are necessary for growth of B.gibsoni, and that the intracellular organelles may be an important factor which enhance the multiplication of B.gibsoni in canine reticulocytes.