Culture of piroplasms using some components of reticulocytes for the purpose of cell-free culture of the parasite.

阐明促进吉氏巴贝斯虫在犬网织红细胞中体外增殖的因素。方法：1.将巴贝斯虫与遗传性高浓度谷氨酸的犬红细胞或低浓度谷氨酸的细胞共同培养。结果：1.高谷氨酸含量的红细胞中寄生虫的增殖率最高。(1)用葡萄糖或谷氨酸培养时，寄生虫的增殖率最高。(2)将寄生虫与含有网织红细胞溶血沉淀物的细胞一起培养时，寄生虫的增殖率增加。(结论)这些结果表明，谷氨酸和葡萄糖都是吉氏巴贝斯虫生长所必需的，胞内细胞器可能是促进犬网织细胞中吉氏巴氏杆菌增殖的重要因素。

To clarify the factors which enhance the multiplication of Babesia gibsoni in canine reticulocytes in vitro.1. Babesia parasites were cultured together with canine erythrocytes with hereditary high concentration of glutamate, or cells with low glutamate.2. Babesia parasites were cultured in the medium containing several materials which present in reticulocytes.(Results)1. The highest multiplication of the parasites was observed in the erythrocytes containing high glutamate.2.(1) A high multiplication of the parasites was observed when the parasites were cultured with glucose or glutamate.(2) The multiplication of the parasites was increased when the parasites were cultured together with resealed cells containing sediments of hemolysate of reticulocytes.(Conclusion)These results indicate that both glutamate and glucose are necessary for growth of B.gibsoni, and that the intracellular organelles may be an important factor which enhance the multiplication of B.gibsoni in canine reticulocytes.