気候変動による熱波が珊瑚礁の魚に及ぼす影響の解明

首先，我是一份出版物的主要作者，在该出版物中，对海葵Amphipron clarkii的染色体规模的基因组进行了测序、生物信息组装和分析。该项目产生了高质量的基因组资源，将用于OIST和世界各地其他研究小组的研究。这些结果发表在《G3：基因|基因组|遗传学》上。我完成了一项实验，调查了海洋变暖对小丑鱼幼体发育的影响。这包括进行一项以水族箱为基础的研究，将幼虫暴露在不同的温度条件下，并记录它们的反应。我发现，幼虫生长发育得更快，代谢率更高，在更高的温度下有许多差异表达的基因。这项实验的结果和随后的生物信息学分析发表在《全环境科学》杂志上。此外，我目前正在完成这项实验的第二份手稿，其中用RNA测序评估了这些幼虫的组织特异性分子反应。这篇论文将于2023年4月提交给《全环境科学》杂志。在幼体实验的基础上，我完成了一项研究海洋热浪对小丑鱼幼鱼影响的实验。这项为期4个月的实验已经完成，并进行了代谢测量和RNA测序采样。基因组数据现在已经收到，正在等待生物信息学分析，分析将于2023年5月完成，然后在2023年7月公布这些结果。

Firstly, I was the lead author on a publication in which the chromosome-scale genome of the anemonefish Amphiprion clarkii was sequenced, bioinformatically assembled and analyzed. The project produced a high-quality genomic resource that will be used for research at OIST and other research groups around the world. These results were published in G3: Genes | Genomes | Genetics.I completed an experiment that investigated the impact of ocean warming on larval development of clownfish. This involved conducting an aquaria-based study in which larvae were exposed to different temperature regimes, and their response recorded. I found that larvae grow and develop much faster, display elevated metabolic rates and have many genes differentially expressed at higher temperatures. The result of this experiment and subsequent bioinformatic analyses were published in the journal Science of the Total Environment. Furthermore, I am currently finalizing the second manuscript from this experiment, in which the tissue-specific molecular response of these larvae was assessed with RNA-sequencing. This paper will be submitted to the journal Science of the Total Environment in April 2023.Following up on the larval experiment I completed an experiment investigating the impact of marine heatwaves on juvenile clownfish. This 4-month long experiment has been completed, with metabolic measurements and sampling for RNA-sequencing conducted. Genomic data has now been received and is awaiting bioinformatic analyses, which will be completed in May 2023, before publication of these results in July 2023.