Functional Analysis of a Novel Obesity-associated E3 Ubiquitin Ligase WWP1

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It is well known that tumor suppressor p53 transcriptionally regulates the expression of several E3 ubiquitin ligases and the ligases post-translationally modulate p53 itself. We found that the expression of p53-targeted E3 ubiquitin ligase WWP1 was specifically up-regulated in white adipose tissue(WAT) of obese mice. An insulin administration reduced the expression of WWP1 protein in WAT in obese mice. Moreover, a knockdown for WWP1 suppressed adipocyte differentiation in 3T3-L1 preadipocyte cell line. Our findings suggest that WWP1 is a novel marker protein of obese WAT, and its expression is regulated by both p53 and insulin.