Functional inhibition of the cardiac costamere induces the formation of contraction bands
心脏肋节的功能抑制诱导收缩带的形成
基本信息
- 批准号:23659205
- 负责人:
- 金额:$ 2.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Challenging Exploratory Research
- 财政年份:2011
- 资助国家:日本
- 起止时间:2011 至 2012
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Contraction band (CB) necrosis, a key morphological hallmark of ischemia-reperfusion injury, is caused by regional contracture at individual sarcomeres of cardiomyocytes. However, mechanisms for CB formation remain undetermined. We hypothesized that regional ablation of β-dystroglycan (βDG) leads to genesis of CBs at the ablated sites. To address this possibility, we applied multiphoton (MP) excitation-evoked CALI (Tanabe et al., Nat Methods 2005) to neonatal rat hearts transfected with cDNA encoding the protein fused with EGFP (βDG-EGFP), where EGFP fluorescence was homogeneously distributed along the cell membrane. MP-excitation of DG-EGFP at a localized area of interest resulted in instantaneous bleach of EGFP, followed by progressive and localized aggregation of actin filaments at the site of irradiation, consistent with CBs In summary, regional ablation of βDG resulted in formation of CB, suggesting an essential role of this protein in formation of CBs.
收缩带坏死是心肌细胞局部肌节挛缩引起的,是缺血再灌注损伤的一个重要形态学标志。然而,炭黑形成的机制仍不确定。我们假设β-三磷酸腺苷(βDG)的局部消融导致了在消融部位发生CBs。为了解决这种可能性,我们将多光子(MP)激发诱发CALI (Tanabe et al., Nat Methods 2005)应用于转染了编码EGFP融合蛋白(βDG-EGFP)的cDNA的新生大鼠心脏,其中EGFP荧光沿细胞膜均匀分布。在局部感兴趣的区域mp激发DG-EGFP导致EGFP的瞬时漂白,随后在照射部位肌动蛋白丝的进行性和局部聚集,与CBs一致。总之,βDG的局部消融导致CB的形成,表明该蛋白在CB的形成中起重要作用。
项目成果
期刊论文数量(11)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Multiphoton excitation-evoked CALI for understanding cardiac pathophysiology
多光子激发诱发的 CALI 用于了解心脏病理生理学
- DOI:
- 发表时间:2012
- 期刊:
- 影响因子:0
- 作者:Tanaka H;Tanabe T;Adachi T;Takahashi M;Yamaoka Y;Dai P;Takamatsu T
- 通讯作者:Takamatsu T
Demonstration of contraction-band formation by multiphoton.
多光子收缩带形成的演示。
- DOI:
- 发表时间:2012
- 期刊:
- 影响因子:0
- 作者:Takahashi M;Tanaka H;Adachi T;Yamaoka Y;Dai P;Takamatsu T.
- 通讯作者:Takamatsu T.
Intrinsic left atrial histoanatomy as the basis for reentrant excitation causing atrial fibrillation/flutter in rats
- DOI:10.1016/j.hrthm.2013.04.021
- 发表时间:2013-09-01
- 期刊:
- 影响因子:5.5
- 作者:Matsuyama, Taka-aki;Tanaka, Hideo;Takamatsu, Tetsuro
- 通讯作者:Takamatsu, Tetsuro
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TAKAMATSU Tetsuro其他文献
TAKAMATSU Tetsuro的其他文献
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{{ truncateString('TAKAMATSU Tetsuro', 18)}}的其他基金
Detection of malignant tumor by using nonlinear Raman spectroscopy
非线性拉曼光谱检测恶性肿瘤
- 批准号:
25560206 - 财政年份:2013
- 资助金额:
$ 2.41万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Visualization of peripheral nerves for nerve-sparing surgery
周围神经的可视化以进行神经保留手术
- 批准号:
24300165 - 财政年份:2012
- 资助金额:
$ 2.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Role of heterocellular gap-junctional communication between cardiomyocytes and myofibroblasts in genesis of ventricular tachy arrhytymias
心肌细胞和肌成纤维细胞之间的异细胞间隙连接通讯在室性快速性心律失常发生中的作用
- 批准号:
20390115 - 财政年份:2008
- 资助金额:
$ 2.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of photoacoustic imaging with near-infrared pulse laser to improve depth resolution
开发近红外脉冲激光光声成像以提高深度分辨率
- 批准号:
18300153 - 财政年份:2006
- 资助金额:
$ 2.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Pathophysiological study of arrhythmogenesis in ischemic heart
缺血性心脏心律失常发生的病理生理学研究
- 批准号:
14370081 - 财政年份:2002
- 资助金额:
$ 2.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Real-time in situ two-photon imaging of intercellular signaling in sinoatrial node
窦房结细胞间信号传导的实时原位双光子成像
- 批准号:
09557022 - 财政年份:1997
- 资助金额:
$ 2.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The Role Of Cardiac Stromal Cells In Arrhythmia
心脏基质细胞在心律失常中的作用
- 批准号:
07457064 - 财政年份:1995
- 资助金额:
$ 2.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Morpho-Physiological Study on Mechanism of Arrhythmia in Heart Muscle Cell by Using High-Speed Image Processor
利用高速图像处理器对心肌细胞心律失常机制进行形态生理学研究
- 批准号:
02670159 - 财政年份:1990
- 资助金额:
$ 2.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)