The Role Of Cardiac Stromal Cells In Arrhythmia

心脏基质细胞在心律失常中的作用

• 批准号: 07457064
• 负责人: TAKAMATSU Tetsuro
• 金额: $ 3.46万
• 依托单位: Kyoto Prefectural University Of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457064/
• 关键词: Cardiomyocyte; Stromal Cell; Calcium Ion; Gap Junction; Calcium wave; Fluo3; Arrhythmia; Confocal Microscopy; フルオ 3; 細胞間伝播

The subcellular mechanisms of fatal arrhythmia such as ventricular flutter and fibrillation are unknown. The purpose of this study is to clarify the role of cardiac stromal cells in these type of arrhythmia. So I investigated regulation of intracellular calcium ion concentration ([Ca^<2+>]_i) in abnormally high [Ca^<2+>]_i and propagation of calcium wave between heart muscle cells and stromal cells. The results obtained in this study are as follow,1) The elevation of diastolic [Ca^<2+>]_i per se directly influenced the [Ca^<2+>]_i-regulation by sarcoplasmic reticulum.2) Confocal images enabled to determine the cell-to-cell junction between clustered heart muscle cells of guinea pig and revealed that the high [Ca^<2+>]_i can evoke calcium-induced calcium release from the sarcoplasmic reticulum in the neighboring heart muscle cells through the gap junction.3) Calcium wave did not propagate between co-cultured heart muscle cells and stromal cells although co-excitation was recognized.4) Ischemia induced temporally abnormal expression of connexin 43 at the edge of the cardiomyocytes bordering the infarcted area.

致命性心律失常如室扑和纤颤的亚细胞机制尚不清楚。本研究的目的是阐明心脏基质细胞在这些类型的心律失常中的作用。因此，我研究了细胞内钙离子浓度([Ca^&lt；2+&gt；]i)在异常高[Ca^&lt；2+&gt；]i中的调节以及钙波在心肌细胞和基质细胞之间的传播。结果如下：1)舒张期[Ca^&lt；2+&gt；]_i升高本身直接影响肌浆网对[Ca^&lt；2+&gt；]i的调节；2)共聚焦图像显示高[Ca^&lt；2+&gt；][_i]_i可通过缝隙连接引起相邻心肌细胞肌浆网钙离子释放。3)共培养的心肌细胞和基质细胞之间没有钙波传播，但存在共刺激。4)缺血诱导梗死区边缘心肌细胞间隙连接蛋白43的表达出现一过性异常。

项目成果

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