Exsome sequencing of world-class sprint athletes

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The purpose of the present study to identify genetic variants associated with anaerobic performance, namely, elite Jamaican (African ancestry) and Japanese sprint athlete status, from analysis of entire exon sequence. Total DNA was isolated from oral mucosa (Jamaican) and saliva (Japanese) with a QIAamp DNA Blood Mini Kit and Oragene DNA Collection Kit, respectively. Entire exon sequencing was analyzed by use of SOLiD4. A lots of sequence errors were found in Jamaican sprinters because of using DNA samples from oral mucosa, but not in Japanese sprinters. Therefore, we excluded in Jamaican athletes from further analysis. In the Japanese cohort, frequencies of the 11 variants were higher Five variants of them cause amino acid replacement. on the other hands, frequencies of the 15 variants were lower in elite Japanese sprinters than controls. Further extensive studies are necessary to confirm or identify genetic variants associated with elite Japanese sprint athlete status.