Search for new target molecules to overcome drug resistance using the Hsp72 binding protein interactome

热休克蛋白72（Hsp 72）具有促进蛋白质折叠、稳定致癌蛋白、避免应激等功能。已有研究表明，胃癌组织中Hsp 72的过表达与化疗耐药有关。本实验采用人胃癌耐药细胞系2 M和奥沙利铂（oxaliplatin，OXA）耐药细胞系2 M（2 M/OXA）。我们通过LC/MS/MS鉴定了22个Hsp 72客户蛋白，其中基质细胞衍生因子2 siRNA可增强OXA诱导的2 M/OXA细胞死亡，耐药细胞中的Hsp 72客户蛋白可能是克服耐药的靶分子。

Heat shock protein 72 (Hsp72) has functions such as promotion of protein folding, stabilizing oncogenic proteins, and avoiding stress. It has reported that Hsp72 overexpressed in gastric cancer was associated with resistance to chemotherapy. We think that Hsp72 client proteins would play an important role in drug resistance.All experiments used human gastric cancer cell line, 2M and oxaliplatin (OXA) resistant 2M (2M/OXA). We identified 22 specific Hsp72-client proteins in 2M/OXA by LC/MS/MS. One of the client proteins, stromal cell derived factor 2 siRNA enhanced OXA inducing cell death in 2M/OXA.Hsp72-client proteins in drug-resistant cells may target molecules to overcome drug resistance.