Structural analysis of microbial loop based on algal organic matter in lake

基于湖泊藻类有机质的微生物循环结构分析

• 批准号: 24651006
• 负责人: KASUGA Ikuro
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24651006/
• 关键词: 安定同位体プローブ法; 湖沼; 藻類産生有機物; 微生物ループ; Limnohabitans; DNA-SIP; FTMS

The structure of microbial loop in lake was evaluated by stable isotope probing. 13C-labelled algal organic matter prepared by cultivating algal pure culture with 13C-bicarbonate was added to lake water. DNA analysis in different density fractions demonstrated that microorganisms related to Limnohabitans predominantly responded to algal organic matter. In addition, flagellates and ciliates also assimilated 13C by preying on Limnohabitans-related bacteria. The close relationship between Limnohabitans-related bacteria and cyanobacteria was observed in the field survey.

利用稳定同位素探针技术对湖泊微生物环的结构进行了评价。将用13 C-碳酸氢盐培养藻类纯培养物制备的13 C-标记藻类有机物添加到湖水中。不同密度组分的DNA分析表明，与湖沼生物相关的微生物主要对藻类有机质做出反应。此外，鞭毛虫和纤毛虫也通过捕食与湖沼生物相关的细菌来同化13 C。在野外调查中发现，与湖泊生境相关的细菌与蓝藻有着密切的关系。

项目成果

春日郁朗のHP

春日郁郎的HP

湖沼微生物ループにおける藻類産生有機物を利用する微生物群の構成と消長

利用湖泊微生物循环中藻类产生的有机物的微生物群落的组成和进化

• 发表时间: 2014
• 作者: 木戸佑樹;春日郁朗;栗栖太;古米弘明
• 通讯作者: 古米弘明