Structural analysis of microbial loop based on algal organic matter in lake

采用稳定同位素探针法对湖泊微生物环路结构进行了评价。将13C-碳酸氢盐纯培养藻类制备的13C标记藻类有机物添加到湖水中。不同密度组分的DNA分析表明，与滩涂相关的微生物主要对藻类有机物做出反应。此外，鞭毛虫和纤毛虫还通过捕食与利姆诺栖动物相关的细菌来吸收13C。在野外调查中观察到淡水栖息地相关细菌与蓝藻的密切关系。

The structure of microbial loop in lake was evaluated by stable isotope probing. 13C-labelled algal organic matter prepared by cultivating algal pure culture with 13C-bicarbonate was added to lake water. DNA analysis in different density fractions demonstrated that microorganisms related to Limnohabitans predominantly responded to algal organic matter. In addition, flagellates and ciliates also assimilated 13C by preying on Limnohabitans-related bacteria. The close relationship between Limnohabitans-related bacteria and cyanobacteria was observed in the field survey.