Search of mutant p53-specific target of cancer cells

寻找突变型 p53 癌细胞特异性靶点

• 批准号: 24650642
• 负责人: ISHIOKA Chikashi
• 金额: $ 2.5万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650642/
• 关键词: 変異p53; 合成致死

This study aimed to find out a synthetic lethal/sickness gene for cancer cells expressing R175H mutant p53, a most frequent mutation in human cancer. A lentiviral shRNA library was transfected in SF126 cells expressing R175H mutant p53 and multiple candidate synthetic lethal/sickness genes against R175H mutant p53 were isolated. One of genes, ID1, truly introduced synthetic lethal/sickness in SF126 by a lentivirus-mediated knockdown by ID1 shRNA. In future, development of ID1 inhibitor might contribute a new era of anti-cancer drugs.

本研究旨在找出表达 R175H 突变体 p53（人类癌症中最常见的突变）的癌细胞的合成致死/疾病基因。将慢病毒 shRNA 文库转染到表达 R175H 突变体 p53 的 SF126 细胞中，并分离出针对 R175H 突变体 p53 的多个候选合成致死/疾病基因。其中一个基因 ID1 通过慢病毒介导的 ID1 shRNA 敲低，真正在 SF126 中引入了合成致死/疾病。未来，ID1抑制剂的开发或将开创抗癌药物的新时代。

项目成果

High throughput RNAi screening of synthetic lethal genes interacting with the common TP53 mutation R175H

高通量 RNAi 筛选与常见 TP53 突变 R175H 相互作用的合成致死基因

• 发表时间: 2012
• 作者: Imai;H.,Kato;S.,Sakamoto;Y.,Takahashi;S.,Kakudo Y.,Shimodaira H.,Ishioka C.
• 通讯作者: S.,Kakudo Y.,Shimodaira H.,Ishioka C.

High throughput RNAi screening identifies ID1 as a synthetic sick/lethal gene interacting with the common TP53 mutation R175H.

• DOI: 10.3892/or.2013.2953
• 发表时间: 2014-03
• 期刊: Oncology reports
• 影响因子: 4.2
• 作者: Imai H;Kato S;Sakamoto Y;Kakudo Y;Shimodaira H;Ishioka C
• 通讯作者: Ishioka C

High throughput RNAi-screening of synthetic sick/lethal genes interacting with the common TP53 mutation R175H.

对与常见 TP53 突变 R175H 相互作用的合成病态/致死基因进行高通量 RNAi 筛选。

• 发表时间: 2012
• 作者: Imai H;et al.
• 通讯作者: et al.