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Construction of a transcriptional-translational regulation platform using a novel poly(A) determination method.

Construction of a transcriptional-translational regulation platform using a novel poly(A) determination method.
使用新型聚腺苷酸测定方法构建转录翻译调控平台。
批准号：
24651212
负责人：
TEI Hajime
金额：
$2.58万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2012
资助国家：
日本
项目状态：
已结题
起止时间：
2012-04-01 至 2013-03-31
来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24651212/
关键词：
概日リズム時計遺伝子視交叉上核Peirod1poly(A)翻訳制御Period1

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中文摘要
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英文摘要
I have constructed a novel method for the determination of poly(A) in eukaryotic mRNA, and designated as PACHINCO RT-PCR. The method was optimized for the application of automatic microchip DNA sequencers, and the elongation of the poly(A) of Per1 clock gene transcripts was clearly determined. Subsequently, the method was applied for NGS sequencers for the improvement of the throughput and analytical precision for poly(A) determination. Accordingly, I have collected mRNAs with the circadian oscillation of poly(A) length in both a rat suprachiasmatic nucleus derived cell line and mouse suprachiasmatic cells.
期刊论文(2)
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科研奖励(0)
会议论文
時計遺伝子、転写・翻訳フィードバックループと概日リズム
时钟基因、转录/翻译反馈环和昼夜节律
DOI： --
发表时间： 2013
期刊：
影响因子： --
作者： [Eriko Kakegawa, Yasuyuki Shimizu, Ichiro Kimura, Yoshihiko Saito and Evgeny Isenko, イエリン賢太郎・斎藤佳彦・木村一郎・大槻政哉・イセンコエフゲニー・清水康行, イエリン賢太郎・斎藤佳彦・木村一郎・大槻政哉・イセンコエフゲニー・清水康行, 程 肇]
通讯作者： 程 肇
Development of translational regulation platform of circadian rhythms using a novel poly(A) determination method
  • 批准号：
    25640100
  • 项目类别：
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额：
    $2.58万
  • 财政年份：
    2013
  • 负责人：
    TEI Hajime
  • 依托单位：
    Kanazawa University
A Genome-wide screening of circadian transcripts using a novel poly(A) determination method
  • 批准号：
    23651184
  • 项目类别：
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额：
    $2.58万
  • 财政年份：
    2011
  • 负责人：
    TEI Hajime
  • 依托单位：
    Kanazawa University
Metabolomics Analysis of Phase Shift and Intercellular Entrainment of Central Circadian Pacemaker Cells
Screening of Molecules for entrainment of circadian rhythms in mammalian central and peripheral clock cells
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