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A Genome-wide screening of circadian transcripts using a novel poly(A) determination method

A Genome-wide screening of circadian transcripts using a novel poly(A) determination method
使用新型 Poly(A) 测定方法对昼夜节律转录本进行全基因组筛选
批准号：
23651184
负责人：
TEI Hajime
金额：
$2.58万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2011
资助国家：
日本
项目状态：
已结题
起止时间：
2011 至 --
来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651184/
关键词：
概日リズム時計遺伝子視交叉上核Periodlpoly(A)翻訳制御

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中文摘要
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英文摘要
We have developed a novel method to determine the length of poly(A) in eukaryotic mRNA, and designated as PACHINCO(Poly(A) Capture by Hairpin Chimeric Oligonucleotide)-RT-PCR. In this year, we optimized the application of the method to an automated microchip electrophoresis system(MultiNA, SHIMADZU), including the development of the distribution analysis algorithm of a variety of poly(A) length.
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会议论文
Positive autoregulation delays the expression phase of mammalian clock gene Per2.
DOI： 10.1371/journal.pone.0018663
发表时间： 2011-04-14
期刊： PloS one
影响因子： 3.7
作者： [Ogawa Y, Koike N, Kurosawa G, Soga T, Tomita M, Tei H]
通讯作者： Tei H
Development of translational regulation platform of circadian rhythms using a novel poly(A) determination method
  • 批准号：
    25640100
  • 项目类别：
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额：
    $2.58万
  • 财政年份：
    2013
  • 负责人：
    TEI Hajime
  • 依托单位：
    Kanazawa University
Construction of a transcriptional-translational regulation platform using a novel poly(A) determination method.
  • 批准号：
    24651212
  • 项目类别：
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额：
    $2.58万
  • 财政年份：
    2012
  • 负责人：
    TEI Hajime
  • 依托单位：
    Kanazawa University
Metabolomics Analysis of Phase Shift and Intercellular Entrainment of Central Circadian Pacemaker Cells
Screening of Molecules for entrainment of circadian rhythms in mammalian central and peripheral clock cells
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