Biosynthetic Studies on Okadaic Acid

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In this study, we tried to identify a gene cluster involved in the biosynthesis of okadaic acid in the dinoflagellate Prorocentrum hoffmanianum. As a result, we succeeded in the PCR amplifications of two PKS genes from the DNA isolated from P. hoffmanianum, and one PKS gene from the chloroplast DNA separated from P. hoffmanianum by using the degenerate primers based on the conserved sequences of bacterial PKSs. We also successfully detected the peptide sequences of the candidate PKSs by LC-orbitrap MS analyses. This is the first detection of a putative PKS proteins from P. hoffmanianum by the proteomic approach. These results will pave the way to future studies to understand the biosynthesis of the complex secondary metabolites in dinoflagellates.