high-speed detection of absolute steric configuration of proteins in solution using chiral-sensitive nonlinear Raman spectroscopy

在本研究中，我们使用了一种新的激光光源，即白光激光光源，开发了一种能够检测分子手性的光谱系统。首先，我们采用光谱干涉技术来提高系统的灵敏度。因此，我们测量了由于手征CARS过程而产生的光电场的实部和虚部。接下来，我们将双色(可见光和近红外)激光脉冲引入光子晶体光纤中，以扩大白光激光的光谱覆盖范围。利用可见光白光激光光源，成功研制了可见光下的CARS-ROA系统，并取得了较好的信噪比。

In the present study, we used a new laser source, namely white-light laser source to develop a spectroscopic system which is capable of detecting molecular chirality. First, we employed spectral interferometric technique to improve the sensitivity of the system. As a result, we measured real and imaginary parts of the optical electric field due to the chiral CARS process. Next, we introduced two-color (visible and near infra-red) laser pulses into a photonic crystal fiber in order to extend spectral coverage of the white-light laser. With the use of the visible white-light laser source, we have successfully developed CARS-ROA system in visible, and found improvement of the signal-to-noise ratio.