Realtime CARS imaging of cellular response by stimulation of laser ablation

我们研制了一套与激光烧蚀系统相结合的多焦点相干反斯托克斯拉曼散射显微系统。所开发的系统通过激光刺激可视化样品响应，而无需以视频速率染色。用于烧蚀的激光束独立于观察而被控制。所开发的系统被应用于细胞响应的膜破裂和结晶监测的光子压力。

We have developed a multifocus coherent anti-Stokes Raman scattering microscopy system combined with a laser-beam ablation system. The developed system visualizes sample response by laser stimulation without staining at vide-rate. The laser beam for ablation is controlled independently of observation. The developed system was applied to cellular response by membrane disruption and crystallizationmonitoring by photon pressure.