Absolute concentration measurement and experimental detection equipment of singlet oxygen on the basis of a new principle

单线态氧是分子氧的最低激发态，是活性氧的代表，因其在许多光敏氧化、光致癌和癌症光动力治疗中的重要作用而备受关注。近年来，人们采用了多种技术来提高探测器的灵敏度。然而，目前使用的技术在单线态氧浓度的定量信息方面存在着重要的局限性，需要克服。值得注意的是，单线态氧的角动量是顺磁性的，基态氧也是顺磁性的。在本工作中，我们观察到了单线态氧的电子顺磁共振(EPR)谱。用基态氧作为定量标准，成功地测定了单线态氧的绝对浓度。我们制作了一套同时观测单线态氧的EPR和近红外发射光谱的实验装置。

Singlet oxygen, the lowest excited state of molecular oxygen, is a representative of reactive oxygen species and is of much interest because of its importance in many photosensitized oxidations, in photocarcinogenisis, and in the photodynamic therapy of cancer. Recently, many kinds of techniques have been employed to improve the sensitivity of the detector. However, the currently used technology has important limitations of quantitative information about singlet oxygen concentration which need to be overcome.It is notable that singlet oxygen is paramagnetic owing to its angular momentum and ground-state oxygen is also paramagnetic. In the present work, we observed the electron paramagnetic resonance (EPR) spectra of singlet oxygen. We have succeeded in determining the absolute concentration of singlet oxygen by using ground-state oxygen as a quantitative standard. We made an experimental equipment to observe the EPR and near-IR emission spectra of singlet oxygen at the same time.