Novel protein reconstitution systems for monitoring protein dynamics in living cells
Novel protein reconstitution systems for monitoring protein dynamics in living cells
批准号:
24655058
负责人:
KANNO Akira
金额:
$2.58万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2014-03-31
中文摘要
在这项研究中,我开发了两种类型的指标来监测活细胞中生物分子的动力学:荧光素酶片段融合到荧光蛋白中,用于高度精确地检测活细胞中磷脂酰肌醇3,4,5-三磷酸(PIP 3)的产生;以及用于高灵敏度实时检测活细胞中半胱天冬酶-3和-8的环状半胱天冬酶。
英文摘要
In this study, I developed two types of indicators to monitor the dynamics of biomolecules in living cells: luciferase fragments fused to a fluorescent protein for the highly precise detection of phosphatidylinositol 3,4,5-trisphosphate (PIP3) production in living cells; and cyclic luciferases for real-time sensing of caspase-3 and -8 in living cells with high sensitivity.
期刊论文(6)
专著(0)
科研奖励(0)
会议论文
エストロゲンを高感度にリアルタイム検出する発光インジケーターの開発
高灵敏实时检测雌激素发光指示剂的研制
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[藤本竜二, 松尾壮, 曽木政伸, 豊田岐聡, 長尾博文, 今岡成章, 早川滋雄, 菅野憲,竹之内修,高倉栄男,小澤岳昌]
通讯作者:
菅野憲,竹之内修,高倉栄男,小澤岳昌
Bioluminescent Probes to Analyze Ligand-induced Phosphatidylinositol 3,4,5-Trisphosphate Production with Split Luciferase Complementation
用于分析配体诱导的磷脂酰肌醇 3,4,5-三磷酸生产与分裂荧光素酶互补的生物发光探针
DOI:
--
发表时间:
2013
期刊:
Anal. Chem.
影响因子:
7.4
作者:
[L.Z. Yang, Y. Nasu, M. Hattori, H. Yoshimura, A. Kanno, and T. Ozawa]
通讯作者:
and T. Ozawa
発光の事典
发光百科全书
DOI:
--
发表时间:
2015
期刊:
影响因子:
--
作者:
[Tatsuya Shoji, Daiki Sugo, Taka-Aki Asoh, Yumi Wakisaka, Kei Murakoshi, Yasuyuki Tsuboi, 伊都将司・宮坂 博]
通讯作者:
伊都将司・宮坂 博
Development of structural colored hydrogel to monitor biomolecules in living organisms.
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批准号:15K05534
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项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.24万
-
财政年份:2015
-
负责人:KANNO Akira
-
依托单位:
Molecular mechanism of floral organogenesis using orchid model plant, Psygmorchis pusilla.
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批准号:22380018
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$12.15万
-
财政年份:2010
-
负责人:KANNO Akira
-
依托单位:
Development of Bioluminescent Probe for Real-Time Imaging ofTernary Complex Formation in Living Cells
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批准号:22750067
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.66万
-
财政年份:2010
-
负责人:KANNO Akira
-
依托单位:
Development of Optical Probe to Identify Proteins Localizing in the Intracellular Juxtamembrane Region
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批准号:20750056
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项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.83万
-
财政年份:2008
-
负责人:KANNO Akira
-
依托单位:
Molecular mechanism of orchid-specific floral organogenesis by a transgenic approach.
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批准号:19380016
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.15万
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财政年份:2007
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负责人:KANNO Akira
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依托单位:
海外基金