Development of Optical Probe to Identify Proteins Localizing in the Intracellular Juxtamembrane Region
Development of Optical Probe to Identify Proteins Localizing in the Intracellular Juxtamembrane Region
批准号:
20750056
负责人:
KANNO Akira
金额:
$2.83万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2009
中文摘要
与细胞运动相关的蛋白质很可能位于细胞内近膜区域。通过使用现有方法,很难识别位于质膜附近的此类蛋白质。在这项研究中,我开发了一种适用于非侵入性鉴定位于质膜或近膜空间中的蛋白质的通用方法。
英文摘要
It is likely that proteins related to cell movement are localized in the intracellular juxtamembrane region. By using existing methods, it is difficult to identify such proteins localized in proximity to the plasma membrane. In this study, I developed a general method applicable to noninvasive identification of proteins localized on the plasma membrane or in the juxtamembrane space.
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DOI:
10.1007/978-1-60327-321-3_9
发表时间:
2009-01-01
期刊:
BIOLUMINESCENCE: METHODS AND PROTOCOLS, SECOND EDITION
影响因子:
--
作者:
[Kanno, Akira, Umezawa, Yoshio, Ozawa, Takeaki]
通讯作者:
Ozawa, Takeaki
生体内のカスパーゼ-3活性を検出する環状ルシフェラーゼの開発
开发环荧光素酶来检测体内 caspase-3 活性
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[菅野憲, 梅澤喜夫, 小澤岳昌]
通讯作者:
小澤岳昌
Bioluminescent Imaging of MAPK Function with Intein-Mediated Reporter Gene Assay
使用内含肽介导的报告基因检测对 MAPK 功能进行生物发光成像
DOI:
--
发表时间:
2009
期刊:
Methods Mol. Biol. 574
影响因子:
--
作者:
[Akira Kanno, Takeaki Ozawa, Yoshio Umezawa]
通讯作者:
Yoshio Umezawa
Bioluminescent Probes to Visualize Biological Functions in Living Cells
生物发光探针可视化活细胞中的生物功能
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Akira Kanno, Naoki Hida, Takeaki Ozawa]
通讯作者:
Takeaki Ozawa
ACTIVATED PROTEASE INDICATOR
活化蛋白酶指示剂
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[]
通讯作者:
共 9 条
Development of structural colored hydrogel to monitor biomolecules in living organisms.
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批准号:15K05534
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.24万
-
财政年份:2015
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负责人:KANNO Akira
-
依托单位:
Novel protein reconstitution systems for monitoring protein dynamics in living cells
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批准号:24655058
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.58万
-
财政年份:2012
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负责人:KANNO Akira
-
依托单位:
Molecular mechanism of floral organogenesis using orchid model plant, Psygmorchis pusilla.
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批准号:22380018
-
项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$12.15万
-
财政年份:2010
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负责人:KANNO Akira
-
依托单位:
Development of Bioluminescent Probe for Real-Time Imaging ofTernary Complex Formation in Living Cells
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批准号:22750067
-
项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.66万
-
财政年份:2010
-
负责人:KANNO Akira
-
依托单位:
Molecular mechanism of orchid-specific floral organogenesis by a transgenic approach.
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批准号:19380016
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.15万
-
财政年份:2007
-
负责人:KANNO Akira
-
依托单位:
海外基金