Development of polymeric macroporous hydrogels for the immobilization of enzymes using an emulsion-gelation method

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Novel polymeric macroporous hydrogels were developed to entrap and immobilize enzymes for improved enzyme reaction process. The emulsion-gelation method using emulsions was used to simultaneously synthesize the gels and entrap enzymes in the randomly distributed, non-interconnected, sphere-like macropores. The lipase, immobilized within the macroporous hydrogel and organogel, successfully catalyzed the hydrolysis in aqueous media and the transesterification in organic media, respectively.