Screening of peptide catalyst by chemically extended molecular evlutionary engineering
Screening of peptide catalyst by chemically extended molecular evlutionary engineering
批准号:
24656510
负责人:
ITO Yoshihiro
金额:
$2.58万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2013-03-31
中文摘要
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英文摘要
Peptide which catalyzes a reaction in organic solvents was screened by chemically extended molecular evolutionary engineering. The target reaction was Aldol condensation which is important for biochemical industry. We prepared two substrates ; one is conjugated with tRNA and another conjugated with biotin molecule for recovery. Peptide catalyst was screened by molecular evolutionary engineering using mRNA display which is tough even in organic solvent due to covalent linking between peptide (phonotype) and mRNA (genotype)
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PEGylated Antibodies and DNA in Organic Media and Genetic PEGylation"in"Green Polymer 2.!!ed. by H. N. Chen and R. A. Gross
有机培养基中的聚乙二醇化抗体和 DNA 以及遗传聚乙二醇化,参见“绿色聚合物 2”。
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[Seiichi Tada, Hiroshi Abe, and Yoshihiro Ito]
通讯作者:
and Yoshihiro Ito
Peroxidase Activity of G-Quadraplex Hemin-Binding DNA Aptamers Determined by Electrochemical Measurement
通过电化学测量测定 G-Quadraplex 氯高铁血红素结合 DNA 适体的过氧化物酶活性
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Kubo I, Hoshino Y, Liu Mingzhe, Abe Hiroshi, Ito Yoshihiro]
通讯作者:
Ito Yoshihiro
PEGylated antibody and DNA in organic media
有机培养基中的聚乙二醇化抗体和 DNA
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Kubo I, Hoshino Y, Liu Mingzhe, Abe Hiroshi, Ito Yoshihiro, Ito Yoshihiro]
通讯作者:
Ito Yoshihiro
DOI:
10.1371/journal.pone.0049235
发表时间:
2012
期刊:
PloS one
影响因子:
3.7
作者:
[Tada S, Andou T, Suzuki T, Dohmae N, Kobatake E, Ito Y]
通讯作者:
Ito Y
Stmcture Formation and Catalytic Activity of DNA in Organic Solvents
DNA 在有机溶剂中的结构形成和催化活性
DOI:
--
发表时间:
2012
期刊:
Angewandte Chemie International Edition
影响因子:
--
作者:
[Hiroshi Abe, Naoko Abe, Aya Shibata, Keiji Ito, Yoshiyuki Tanaka, Mika Ito, Hisao Saneyoshi, Satoshi Shuto, and Yoshihiro Ito]
通讯作者:
and Yoshihiro Ito
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