Screening of peptide catalyst by chemically extended molecular evlutionary engineering

化学延伸分子进化工程筛选肽催化剂

• 批准号: 24656510
• 负责人: ITO Yoshihiro
• 金额: $ 2.58万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24656510/
• 关键词: 分子進化工学; mRNAディスプレイ; ペプチド触媒; ミスアシル化tRNA; アルドール縮合; 進化分子工学; 非天然アミノ酸

Peptide which catalyzes a reaction in organic solvents was screened by chemically extended molecular evolutionary engineering. The target reaction was Aldol condensation which is important for biochemical industry. We prepared two substrates ; one is conjugated with tRNA and another conjugated with biotin molecule for recovery. Peptide catalyst was screened by molecular evolutionary engineering using mRNA display which is tough even in organic solvent due to covalent linking between peptide (phonotype) and mRNA (genotype)

利用化学扩展分子进化工程技术筛选了在有机溶剂中催化反应的多肽。目标反应是醛醇缩合反应，这在生化工业中具有重要意义。我们制备了两种底物；一种与tRNA结合，另一种与生物素分子结合用于恢复。采用分子进化工程技术筛选多肽催化剂，利用mRNA展示技术，通过多肽（音型）和mRNA（基因型）之间的共价连接，使多肽催化剂在有机溶剂中也具有较强的稳定性。

项目成果

PEGylated Antibodies and DNA in Organic Media and Genetic PEGylation"in"Green Polymer 2.!!ed. by H. N. Chen and R. A. Gross

有机培养基中的聚乙二醇化抗体和 DNA 以及遗传聚乙二醇化，参见“绿色聚合物 2”。

• 发表时间: 2013
• 作者: Seiichi Tada;Hiroshi Abe;and Yoshihiro Ito
• 通讯作者: and Yoshihiro Ito

Peroxidase Activity of G-Quadraplex Hemin-Binding DNA Aptamers Determined by Electrochemical Measurement

通过电化学测量测定 G-Quadraplex 氯高铁血红素结合 DNA 适体的过氧化物酶活性

• 发表时间: 2012
• 作者: Kubo I;Hoshino Y;Liu Mingzhe;Abe Hiroshi;Ito Yoshihiro
• 通讯作者: Ito Yoshihiro

PEGylated antibody and DNA in organic media

有机培养基中的聚乙二醇化抗体和 DNA

• 发表时间: 2012
• 作者: Kubo I;Hoshino Y;Liu Mingzhe;Abe Hiroshi;Ito Yoshihiro;Ito Yoshihiro
• 通讯作者: Ito Yoshihiro

Genetic PEGylation.

• DOI: 10.1371/journal.pone.0049235
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Tada S;Andou T;Suzuki T;Dohmae N;Kobatake E;Ito Y
• 通讯作者: Ito Y

Stmcture Formation and Catalytic Activity of DNA in Organic Solvents

DNA 在有机溶剂中的结构形成和催化活性

• 发表时间: 2012
• 期刊: Angewandte Chemie International Edition
• 作者: Hiroshi Abe;Naoko Abe;Aya Shibata;Keiji Ito;Yoshiyuki Tanaka;Mika Ito;Hisao Saneyoshi;Satoshi Shuto;and Yoshihiro Ito
• 通讯作者: and Yoshihiro Ito