Analysis of effect of autologou bone marrow transplantation in neurogenic pain model

自体骨髓移植在神经源性疼痛模型中的作用分析

• 批准号: 17500360
• 负责人: ITO Yoshihiro
• 金额: $ 2.3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500360/
• 关键词: rehabilitation; transplantation; tissue engineering; neuroscience

Complex regional pain syndrome develops after noxious events such as sprains, various injuries, fracture, nerve lesions or surgery. It is characterized by severe pain, allodynia, edema, discoloration and autonomic disorders. Details of the mechanism remain unclear. Bone marrow transplantation promotes angiogenesis in the lower leg with arteriosclerosis obliterans and reduces severe pain for a few days. We therefore analyzed the effect of autologous bone marrow transplantation in the rat model of neurogenic pain that is usually used to study complex regional pain syndrome.We initially applied an immunohistochemical stain to detect calcitonin gene-related peptide and substance-P in the spinal cord and muscle of affected paws and then compared pain thresholds between Sprague-Dawley and Wistar Lewis rats. We improved the coated wire mesh floor of the cages and constructed a pain behavior chart that originally comprised 12 items. Thereafter, bone marrow from another Lewis rat was transplanted into a 7-week-old model of chronic contraction injury (n=7), and cultured mesenchymal stem cells transplanted from Lewis rats into another group (n=7). The control group comprised chronic contraction injured Lewis rats (n=7). The results were not statistically related among the three groups, changes in pain behavior were transient. And immunohistochemical changes were absent. In following year, we transplanted bone marrow into the subarachnoid space of the 7-week-old model of chronic contraction injury and found an increased pain threshold on the first post-operative day. Statistical significance was difficult to ascertain because the number of rats was too small.We therefore plan to explore these transplantation methods in a larger number of rats.

在扭伤、各种损伤、骨折、神经损伤或手术等有害事件后，会出现复杂的局部疼痛综合征。它的特点是剧烈疼痛、异常性疼痛、水肿、变色和自主神经紊乱。该机制的细节尚不清楚。骨髓移植促进下肢动脉硬化闭塞的血管生成，并减轻几天的剧烈疼痛。因此，我们分析了自体骨髓移植对神经源性疼痛大鼠模型的影响，该模型通常用于研究复杂的区域性疼痛综合征。我们首先应用免疫组织化学染色检测患爪脊髓和肌肉中降钙素基因相关肽和p物质，然后比较Sprague-Dawley和Wistar Lewis大鼠的疼痛阈值。我们改进了笼子的涂层铁丝网地板，并构建了一个最初包含12个项目的疼痛行为图表。随后，将另一只Lewis大鼠骨髓移植至7周龄慢性收缩损伤模型（n=7），将Lewis大鼠培养的间充质干细胞移植至另一组（n=7）。对照组为慢性收缩损伤Lewis大鼠（n=7）。结果在三组之间无统计学相关性，疼痛行为的改变是短暂的。未见免疫组化变化。第二年，我们将骨髓移植到7周大的慢性收缩性损伤模型的蛛网膜下腔，发现术后第一天疼痛阈值升高。由于大鼠数量太少，统计意义难以确定。因此，我们计划在更多的大鼠中探索这些移植方法。