The analysis of copy number variations in the field populations for disease ecology

疾病生态学领域野外种群拷贝数变异分析

• 批准号: 24657011
• 负责人: KAWATA Masakado
• 金额: $ 2.66万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657011/
• 关键词: 分子生態; 疾病生態学; 保全生態学; 抗菌ペプチド; 遺伝子コピー数変異; 遺伝子コピー数; ヤマアカガエル

Our previous study indicated that anti peptide, Temporin-1 in Rana ornativentirs shows copy-number variations. In order to determine DNA sequences in the regions including temporin-1, we created fosmid library and chose the clone libraries including temporin-1. Then we tried to determine these DNA sequences using the next generation sequencer (Ion PGM). The results showed that among about 40kbp DNA region, those in 33kbp were non-coding regions. The rest of the sequences contained at least three copies of temporin-1, including temporin-1Oj and temproin-1OC. This suggests that these copies of temporin might be tenderly repeated. At the present study, we could not determine complete sequences of these regions.

我们先前的研究表明，华东林蛙的抗肽--时间蛋白-1存在拷贝数变异。为了确定包括Temporin-1在内的区域的DNA序列，我们建立了Fosmid文库，并选择了包括Temporin-1在内的克隆文库。然后，我们试图使用下一代测序仪(Ion PGM)来确定这些DNA序列。结果表明，在约40kbpDNA片段中，33kbpDNA片段为非编码区。其余的序列包含至少三个拷贝的Temporin-1，包括Temproin-10J和Temproin-10C。这表明，这些临时蛋白的拷贝可能会被温柔地重复。在目前的研究中，我们不能确定这些区域的完整序列。

项目成果

Genome-wide deserts for copy number variation in vertebrates

• DOI: 10.1038/ncomms3283
• 发表时间: 2013-08-01
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Makino, Takashi;McLysaght, Aoife;Kawata, Masakado
• 通讯作者: Kawata, Masakado