Innovative environmental DNA technique for community ecology

社区生态的创新环境DNA技术

• 批准号: 24657020
• 负责人: KONDOH Michio
• 金额: $ 2.16万
• 依托单位: Ryukoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657020/
• 关键词: 環境DNA; 魚類群集; DNAバーコーディング; 環境モニタリング; 琵琶湖水系; 群集ネットワーク

We have developed the molecular and theoretical techniques to establish eDNA-based fish community monitoring. The major achievements are as follows: (i) A molecular biological methodology established to detect and quantify eDNA of seven fish species of the Lake Biwa-Yodo River System; (ii) DNA release and decomposition rates of fish individual, the basic parameters, and their condition dependence experimentally determined for particular species; (iii) Field and laboratory experiments conducted to test the methods developed for eDNA-based monitoring of bluegill and Japanese giant salamander; (iv) Novel methodologies established to capture and concentrate eDNA in water samples; (v) Multispecies detection technique based on multiplex PCR studied; (vi) Novel theoretical hypotheses obtained as to community network structure and its community dynamics consequences.

我们已经开发了分子和理论技术，以建立基于eDNA的鱼类群落监测。主要成果如下：（i）建立了一种检测和定量测定琵琶湖-淀川水系7种鱼类eDNA的分子生物学方法;（ii）通过实验确定了特定鱼类个体DNA释放和分解速率、基本参数及其条件依赖性; ㈢进行实地和实验室实验，以测试为利用脱氧核糖核酸监测蓝鳃金龟和日本大鲵而开发的方法;（v）研究了基于多重PCR的多物种检测技术;（vi）获得了关于群落网络结构及其群落动态后果的新理论假说。

项目成果

Using environmental DNA to estimate the distribution of an invasive fish species in ponds.

• DOI: 10.1371/journal.pone.0056584
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Takahara T;Minamoto T;Doi H
• 通讯作者: Doi H

Hypoxic conditions enhance refuge effect of macrophyte zone for small prey fish from piscivorous predators

缺氧条件增强了水生植物区对小型被捕食鱼的避难效果

• DOI: 10.1111/fme.12033
• 发表时间: 2013
• 期刊: Fisheries Management and Ecology
• 影响因子: 2
• 作者: Yamanaka;H.
• 通讯作者: H.

Large scale environmental DNA assessment for Japanese and Chinese giant salamanders in Katsura River, Japan

日本桂川日本和中国大鲵的大规模环境 DNA 评估

• 发表时间: 2014
• 作者: Fukumoto;S.;Ushimaru;A.;Minamoto;T.
• 通讯作者: T.

同種内外来種の侵入規模の迅速把握

快速了解同一物种内外来物种的入侵规模

• 发表时间: 2014
• 作者: 内井喜美子;土居秀幸;源利文;山中裕樹
• 通讯作者: 山中裕樹

環境DNAを用いた水中生物モニタリングの現状

利用环境DNA监测水生生物的现状

• 发表时间: 2013
• 作者: Kondoh;M.;源利文
• 通讯作者: 源利文