Identification of genes for enzymes involved in betalain biosynthesis and expression analysis in non-Dianthus plants.

点击翻译按钮获取中文摘要

In order to elucidate the unidentified enzymes including betalain synthesis, the sepals of four o'clock were analyzed by RNA-seq. Nine candidate cDNAs for the enzyme catalyzing tyrosine to L-DOPA, which was the first key step enzyme of betalain synthesis, were identified. The genomic structure of one of them, CYP76AD3, which was homolog of CYP76AD1 derived from red beet, was revealed. In the CYP76AD3 gene of variegated sepals with red sectors or dots on a yellow background, CACTA transposable element, dTmj1, was inserted in the intron of CYP76AD3 gene to disturb its transcription, resulting to prevent the expression of CYP76AD3 activity or red betacyanin synthesis, but to synthesize yellow betaxanthins. The movement of dTmj1 in this variegated sepals caused to revert the gene activity and the synthesis of betacyanin revived to generate red sectors or dots on a yellow background.