Measurement of cAMP concentration in a single neuron associated with learning

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It has been hypothesized that cAMP concentration would be increased in a presynaptic neuron in the brain during the learning process. However, nobody has succeeded in measuring this cAMP concentration in a single neuron. To address this issue, we attempted to develop the measurement system in the key single neuron of the pond snail Lymnaea stagnalis. When we applied the super-sensitive measurement method by combination of an ELISA method and an enzymatic cycling method, we clarified that the cAMP concentration can be measured in a single neuron. We should continue to examine the change in this cAMP concentration in a single neuron in the brain by a learning procedure.