Measurement of cAMP concentration in a single neuron associated with learning

测量与学习相关的单个神经元中的 cAMP 浓度

• 批准号: 24657055
• 负责人: ITO Etsuro
• 金额: $ 2.58万
• 依托单位: Tokushima Bunri University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657055/
• 关键词: 学習; 記憶; 環状アデノシン一リン酸; cAMP; 単一細胞; 学習記憶; モノアラガイ

It has been hypothesized that cAMP concentration would be increased in a presynaptic neuron in the brain during the learning process. However, nobody has succeeded in measuring this cAMP concentration in a single neuron. To address this issue, we attempted to develop the measurement system in the key single neuron of the pond snail Lymnaea stagnalis. When we applied the super-sensitive measurement method by combination of an ELISA method and an enzymatic cycling method, we clarified that the cAMP concentration can be measured in a single neuron. We should continue to examine the change in this cAMP concentration in a single neuron in the brain by a learning procedure.

据推测，在学习过程中，大脑中突触前神经元的cAMP浓度会增加。然而，没有人成功地测量了单个神经元中的cAMP浓度。为了解决这一问题，我们试图在池蜗牛的关键单神经元的测量系统的发展停滞。当我们通过ELISA方法和酶循环方法的组合应用超灵敏测量方法时，我们阐明了可以在单个神经元中测量cAMP浓度。我们应该继续通过学习过程来检查大脑中单个神经元中cAMP浓度的变化。

项目成果

What are the elements of motivation for acquisition of conditioned taste aversion?

• DOI: 10.1016/j.nlm.2013.10.013
• 发表时间: 2014-01-01
• 期刊: NEUROBIOLOGY OF LEARNING AND MEMORY
• 影响因子: 2.7
• 作者: Mita, Koichi;Okuta, Akiko;Ito, Etsuro
• 通讯作者: Ito, Etsuro

Paired pulse ratio analysis of insulin-induced synaptic plasticity in the snail brain

胰岛素诱导的蜗牛脑突触可塑性的配对脉冲比分析

• 发表时间: 2013
• 期刊: J. Exp. Biol.
• 作者: J.Murakami;R.Okada;Y.Fujito;M.Sakakibara;K.Lukowiak and E.Ito
• 通讯作者: K.Lukowiak and E.Ito

GABAergic effects on the slow oscillatory neural activities in the procerebrum of Limax valentianus

GABA能对华氏蜗牛前脑慢振荡神经活动的影响

• 发表时间: 2012
• 期刊: Acta Biologica Hungarica
• 作者: Kobayashi S;Ito E
• 通讯作者: Ito E

Increase in cyclic AMP concentration in a cerebral giant interneuron mimics part of a memory trace for conditioned taste aversion of the pond snail.

• DOI: 10.2142/biophysics.9.161
• 发表时间: 2013-01-01
• 期刊: Biophysics (Nagoya-shi, Japan)
• 作者: Otsuka, Emi;Matsunaga, Miho;Ito, Etsuro
• 通讯作者: Ito, Etsuro

Involvement of Insulin-Like Peptide in Long-Term Synaptic Plasticity and Long-Term Memory of the Pond Snail Lymnaea stagnalis

• DOI: 10.1523/jneurosci.0679-12.2013
• 发表时间: 2013-01-02
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Murakami, Jun;Okada, Ryuichi;Ito, Etsuro
• 通讯作者: Ito, Etsuro