Megakaryocytic differentiation of hematopoietic stem cell by NF-E2 transcription factors.
NF-E2转录因子对造血干细胞的巨核细胞分化。
基本信息
- 批准号:08670847
- 负责人:
- 金额:$ 1.6万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
NF-E2 is a heterodimer composed of a lineage-specific subunit p45 and a ubiquitous subunit p18 which belongs to small Maf family. In order to understand the role of NF-E2 transcription factors in megakaryocytic differention of hematopoietic stem cells, we have studied the expression of the transcription factors in purified early hematopoietic progenitor cells (HPCs) induced to unilineage megakaryocytic differentiation in a liquid culture system by reverse transcriptase mediated polymerase chain reaction (RT-PCR). Expression of p45 NF-E2 and its related transcription factors, Nrf1 and Nrf2, was induced during megakaryocytic differentiation. Furthermore, we found that p18 (mafK) and mafG were also induced during megakaryocytic differention using chronic myelogenous leukemia (CML) blasts. Especially expression of mafG was induced more abundantly. These results indicated that Nrf1 and Nrf2 as well as p45 play an important role in megakaryocytic differentiation as heterodimers with small Mafs.We next studied the possibility that Nrf1 and Nrf2 from heterodimers with small Mafs. We construct prokaryotic expression plasmids for mafK,mafG,p45, Nrf1 and Nrf2. Recombinant proteins were purified with Ni-NTA regin and Electrophoretic gel mobility shift assays (EGMSA) was performed. EGMSA showed that small Maf proteins form heterodimers with Nrf1 and Nrf2 and recognize the NF-E2 motif.
NF-E2是一种异源二聚体,由一个谱系特异性亚基p45和一个普遍存在的亚基p18组成,属于小的Maf家族。为了了解NF-E2转录因子在造血干细胞巨核细胞分化中的作用,我们利用逆转录酶介导的聚合酶链反应(RT-PCR)研究了纯化的早期造血祖细胞(HPCs)在液体培养系统中诱导单系巨核细胞分化的转录因子表达。在巨核细胞分化过程中,诱导p45 NF-E2及其相关转录因子Nrf1和Nrf2的表达。此外,我们发现p18 (mafK)和mafG在慢性髓性白血病(CML)巨核细胞分化过程中也被诱导。特别是诱导了更丰富的mafG表达。这些结果表明Nrf1和Nrf2以及p45作为异源二聚体在巨核细胞分化中发挥重要作用。接下来,我们研究了Nrf1和Nrf2来自具有小maf的异源二聚体的可能性。我们构建了mafK、mafG、p45、Nrf1和Nrf2的原核表达质粒。重组蛋白用Ni-NTA区纯化,并进行电泳凝胶迁移量测定(EGMSA)。EGMSA显示小Maf蛋白与Nrf1和Nrf2形成异源二聚体,并识别NF-E2基序。
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Toki,T.,et al.: "Abundant expression of erythroid transcription factor p45NF-E2 mRNA in human peripheral granulocytes." Biochem.Bioph.Res.Co.219. 760-765 (1996)
Toki,T.,et al.:“人外周粒细胞中红系转录因子 p45NF-E2 mRNA 的大量表达。”
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- 影响因子:0
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Toki T, Ito E et al.: "Human small Maf proteins form heterodimers with CNC family transcription factors and recognize the NF-E2 motif." Oncogene. 14. 1901-1910 (1997)
Toki T、Ito E 等人:“人类小 Maf 蛋白与 CNC 家族转录因子形成异二聚体,并识别 NF-E2 基序。”
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- 影响因子:0
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Hirai H,Shimazaki C,Yamagata N,Goto H,Inaba T,Kikuta T,Sumikuma T,Sudo Y,Ashihara E,Fujita N,Hibi S,Imashuku S,Ito E,Nakagawa M.: "Effects of thrombopoietin (c-mpl ligand) on growth ofblast cells from patients with transient abnormal myelopoiesis and acut
Hirai H,Shimazaki C,Yamagata N,Goto H,Inaba T,Kikuta T,Sumikuma T,Sudo Y,Ashihara E,Fujita N,Hibi S,Imashuku S,Ito E,Nakakawa M.:“血小板生成素的影响(c-
- DOI:
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- 影响因子:0
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Toki T,Itoh J,Kitazawa J,Arai K,Yokoyama M,Igarashi K,Yamamoto M and Ito E.: "Abundant expression of erythroid transcription factor p45 NF-E2 mRNA in human peripheral granulocytes." Biochem.Biophys.Res.Commun.219. 760-765 (1996)
Toki T、Itoh J、Kitazawa J、Arai K、Yokoyama M、Igarashi K、Yamamoto M 和 Ito E.:“人外周粒细胞中红系转录因子 p45 NF-E2 mRNA 的丰富表达。”
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- 影响因子:0
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Toki T,Itoh J,Kitasawa J,Arai K,Hatakeyama K,Akasaka J,Igarashi K,Nomura N,Yokoyama M,Yamamoto M and Ito E: "Human small Maf proteins form heterodimers with CNC family transcripition factors and recognize the NF-E2 motif." Oncogene. 14. 1901-1910 (1997)
Toki T、Itoh J、Kitasawa J、Arai K、Hatakeyama K、Akasaka J、Igarashi K、Nomura N、Yokoyama M、Yamamoto M 和 Ito E:“人类小 Maf 蛋白与 CNC 家族转录因子形成异二聚体并识别 NF-
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{{ truncateString('ITO Etsuro', 18)}}的其他基金
Measurement of cAMP concentration in a single neuron associated with learning
测量与学习相关的单个神经元中的 cAMP 浓度
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24657055 - 财政年份:2012
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
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GATA1突变导致短暂性白血病的分子机制及分子靶向治疗的进展
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20390289 - 财政年份:2008
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Behavioral changes clarified from transcriptional and translational mechanisms in a single neuron of a snail
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19370030 - 财政年份:2007
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The molecular mechanisms of leukmogenesis by GATA1
GATA1导致白血病发生的分子机制
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17390295 - 财政年份:2005
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Molecular mechanisms of multi-step leukemogenesis in Down syndrome
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17013004 - 财政年份:2005
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Grant-in-Aid for Scientific Research on Priority Areas
Relation between change in animal behavior and change in transcription factor in a single neuron
动物行为变化与单个神经元转录因子变化之间的关系
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16370033 - 财政年份:2004
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福克斯唐氏综合症相关急性巨核细胞白血病致病基因的鉴定
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14370238 - 财政年份:2002
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Study for the mechanism of lymphocyte development by Bach2 transcription factor and clinical analysis of Bach2 gene
Bach2转录因子淋巴细胞发育机制研究及Bach2基因临床分析
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12670721 - 财政年份:2000
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Grant-in-Aid for Scientific Research (C)
Study for the mechanism of lymphocyte development by NF-E2 transcription factors and clinical analysis of NF-E2 gene.
NF-E2转录因子影响淋巴细胞发育的机制研究及NF-E2基因临床分析。
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10670700 - 财政年份:1998
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$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
New classification of infantile leukemia using lineage-specific transcription
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- 批准号:
06670759 - 财政年份:1994
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$ 1.6万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
ACETYLATION OF P45/NF E2 BY PCAF AND ITS CONSEQUENCES
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