Experimental development a new cryo-holder suitable to electron cryo-tomography

实验开发一种适用于电子冷冻断层扫描的新型冷冻支架

• 批准号: 24657075
• 负责人: YASUNAGA Takuo
• 金额: $ 2.58万
• 依托单位: Kyushu Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657075/
• 关键词: クライオ電子顕微鏡; 構造生物学; 電子顕微鏡法; 試料ホルダー; 蛋白質構造解析

The new cryo-holder suited to electron cryo-tomography was planed by using low-vibration cryo-pump and has been developed. Furthermore, conventional liquid-nitrogen-type cryo-holder was tested and successfully achieved a lower-temperature stage than conventional one by drawing vacuum with vacuum pumps. Hoses for vacuum drawing were important for less vibration of the stage and better hoses give the stable stage to obtain electro micrographs. Currently we are planning to develop a new holder by combining both techniques.

采用低振动低温泵设计并研制了适用于电子层析成像的新型低温保持架。此外，对传统液氮型低温保持器进行了实验，通过真空泵抽真空，成功地实现了比传统液氮型低温保持器更低的温度。用于真空抽吸的软管对于减少载物台的振动是重要的，并且更好的软管提供稳定的载物台以获得电子显微照片。目前，我们正计划通过结合这两种技术开发一种新的保持器。

项目成果

Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans.

• DOI: 10.7554/elife.00133
• 发表时间: 2013-03-06
• 期刊: eLife
• 影响因子: 7.7
• 作者: Yan J;Chao DL;Toba S;Koyasako K;Yasunaga T;Hirotsune S;Shen K
• 通讯作者: Shen K

安永研究室HP

安永研究所HP

Tilt-angle measurement of a sample stage using a capacitive liquid-based inclinometer

使用电容式液基倾角仪测量样品台的倾斜角

• DOI: 10.1093/jmicro/dfs034
• 发表时间: 2012
• 期刊: J.Electron.Microsc.
• 作者: M.Matoba;et al
• 通讯作者: et al