Microtubule-dependent dorsal determination in vertebrate embryogenesis

脊椎动物胚胎发生中微管依赖性背侧决定

• 批准号: 24657152
• 负责人: HIBI Masahiko
• 金额: $ 2.66万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657152/
• 关键词: 体軸形成; ゼブラフィッシュ; 胚発生; 遺伝学; 微小管; 背側決定; モータータンパク質; Wntシグナル; 変異体; 小胞輸送

In amphibian and teleost embryos, dorsal determinants are initially localized to the vegetal pole and then transported to the dorsal side of the embryo along a microtubule array. The dorsal determinants activate the canonical Wnt pathway that is required for the formation of dorsal tissues. Using transgenic zebrafish lines expressing GFP-Tubulin and EB1-GFP, we found that a parallel microtubule array formed at the vegetal pole. The plus end of microtubule array was directed to the future dorsal side. The microtubules themselves moved to the future dorsal side and the vesicles moved along with vegetal microtubules. We found that Syntabulin, which is required for dorsal determination, surrounded vesicles and moved along with them to the dorsal side. Syntabulin interacted with Grip2a and Wntelss. Our data suggest that Syntabulin interacts with Grip2a and Wntless to recruit the vesicles containing the dorsal determinants onto the microtubules to transport them to the dorsal side.

在两栖类和硬骨鱼类胚胎中，背侧决定簇最初定位于植物极，然后沿着微管阵列运输到胚胎的背侧。背侧决定子激活背侧组织形成所需的经典Wnt途径。利用表达GFP-Tubulin和EB 1-GFP的转基因斑马鱼品系，我们发现在植物极形成了平行的微管阵列。微管阵列的正端指向未来的背侧。微管本身移动到未来的背侧和囊泡移动沿着与植物微管。我们发现，Syntabulin，这是所需的背决定，包围囊泡和移动沿着与他们的背侧。Syntabulin与Grip 2a和Wntelss相互作用。我们的数据表明，Syntabulin与Grip 2a和Wntless相互作用，将含有背侧决定簇的囊泡募集到微管上，将其运输到背侧。

项目成果

名古屋大学生物機能開発利用研究センター動物器官機能研究分野

名古屋大学生物功能开发利用研究中心动物器官功能研究部

Syntabulin-mediated transport of Wnt signaling machinery in zebrafish dorsal determination

斑马鱼背侧测定中合成蛋白介导的 Wnt 信号机制转运

• 发表时间: 2012
• 作者: Seki R;Hino H;Takeda S;Pelegri F;Hibi M.
• 通讯作者: Hibi M.

真骨魚類における小脳神経回路形成

硬骨鱼小脑神经回路的形成

• 作者: Hino H;Seki R;Shimizu T;Pelegri F;Hibi M.;日比正彦;日比正彦
• 通讯作者: 日比正彦

Sox5 functions as a fate switch in medaka pigment cell development.

• DOI: 10.1371/journal.pgen.1004246
• 发表时间: 2014-04
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Nagao Y;Suzuki T;Shimizu A;Kimura T;Seki R;Adachi T;Inoue C;Omae Y;Kamei Y;Hara I;Taniguchi Y;Naruse K;Wakamatsu Y;Kelsh RN;Hibi M;Hashimoto H
• 通讯作者: Hashimoto H

Moving microtubule-mediated dorsal determination

移动微管介导的背侧测定

• 发表时间: 2013
• 作者: Hino H;Seki R;Shimizu T;Pelegri F;Hibi M.
• 通讯作者: Hibi M.