Mechanisms of axis formation during early vertebrate embryogenesis
脊椎动物早期胚胎发生过程中轴形成的机制
基本信息
- 批准号:13680805
- 负责人:
- 金额:$ 2.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In amphibian and teleost embryogenesis, the program initiating axis formation begins soon after fertilization. In these species, the dorsal determinants are believed to be initially localized to the vegetal pole, and then transported to the dorsal side of the embryo. The molecular nature of the dorsal determinants has not been elucidated. It is known that these molecules activate the canonical Wnt pathway, leading to the nuclear accumulation of β-catenin on dorsal side, which further stimulates the expression of genes involved in the dorsal organizer induction. The zebrafish homeobox gene bozozok acts downstream of the Wnt signaling pathway and is required for organizer induction. Bozozok functions as a transcriptional repressor and inhibits dorsal expression of a novel bomeobox gene ved. Ved also functions as a transcriptional repressor that represses organizer genes. We further found that ved functions redundantly with vox and vent to restrict the organizer domain. In this study, we also isolated the gene responsible for zebrafish ventralized mutant ogon by a positional cloning strategy and found that the ogon locus encodes a zebrafish homologue of Sizzled. Ogon/Sizzled functions as a negative feedback regulator of BMP signaling. In addition, we generated mice deficient in fez and fez-like, which are expressed specifically in forebarin during early embryogenesis.
在两栖动物和硬骨鱼的胚胎发生中,启动轴形成的程序在受精后不久开始。在这些物种中,背侧决定因子被认为最初定位于植物极,然后转移到胚胎的背侧。背侧决定因素的分子性质尚未阐明。已知这些分子激活了典型的Wnt通路,导致β-catenin在背侧的核积累,进一步刺激了参与背侧组织者诱导的基因的表达。斑马鱼同源盒基因bozozok作用于Wnt信号通路的下游,是组织者诱导所必需的。Bozozok是一种转录抑制因子,可抑制一种新型基因组盒基因的背侧表达。Ved还作为一种转录抑制因子,抑制组织者基因。我们进一步发现,使用冗余的vox和vent函数来限制组织者域。在本研究中,我们还通过定位克隆策略分离了斑马鱼腹化突变体ogon的基因,发现该基因座编码斑马鱼sizzed的同源基因。Ogon/ sizzed作为BMP信号的负反馈调节因子。此外,我们制备了fez和fez样蛋白缺乏的小鼠,这两种蛋白在胚胎发生早期特异性表达于前脑素中。
项目成果
期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Levkowitz, G. Zeller, J., Sirotkin, H.I., French, D., Schilbach, S., Hashimoto, H., Hibi, M., Talbot, W.S., and Rosenthal, A.: "Zinc finger protein too few controls the development of monoaminergic neurons"Nature Neuroscience,. 6. 28-33 (2003)
Levkowitz, G. Zeller, J.、Sirotkin, H.I.、French, D.、Schilbach, S.、Hashimoto, H.、Hibi, M.、Talbot, W.S. 和 Rosenthal, A.:“锌指蛋白对照太少
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Shimizu, T., Yamanaka, Y., Nojima, H., Yabe, T., Hibi, M., Hirano, T.: "A novel repressor-type homeobox gene, ved, is involved in dharma/bozozok-mediated dorsal organizer formation in zebrafish"Mechanisms of Development. 118. 125-138 (2002)
Shimizu, T.、Yamanaka, Y.、Nojima, H.、Yabe, T.、Hibi, M.、Hirano, T.:“一种新型阻遏型同源盒基因 ved,参与 dharma/bozozok 介导的背侧
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Ryu, S-L., Fujii, R., Yamanaka, Y., Shimizu, T., Yabe, T., Hirata, T., Hibi, M., Hirano, T.: "Regulation of dharma/bozozok by the Wnt pathway"Developmental Biology. 231. 397-409 (2001)
Ryu, S-L.、Fujii, R.、Yamanaka, Y.、Shimizu, T.、Yabe, T.、Hirata, T.、Hib, M.、Hirano, T.:“Wnt 途径对 dharma/bozozok 的调节
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Ryu, S-L., Fujii, R., Yamanaka, Y., Shimizu, T., Yabe, T., Hirata, T., Hibi, M., Hirano, T: "Regulation of dharma/bozozok by the Wnt pathway"Development Biology. 231. 397-409 (2001)
Ryu, S-L.、Fujii, R.、Yamanaka, Y.、Shimizu, T.、Yabe, T.、Hirata, T.、Hib, M.、Hirano, T:“Wnt 途径对 dharma/bozozok 的调节”
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Ryu, S-L, et al.: "Regulation of dharma/bozozok by the Wnt signal"Developmental Biology. 231. 397-409 (2001)
Ryu, S-L 等人:“Wnt 信号对 dharma/bozozok 的调节”发育生物学。
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HIBI Masahiko其他文献
HIBI Masahiko的其他文献
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{{ truncateString('HIBI Masahiko', 18)}}的其他基金
Microtubule-dependent dorsal determination in vertebrate embryogenesis
脊椎动物胚胎发生中微管依赖性背侧决定
- 批准号:
24657152 - 财政年份:2012
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Screening of maternal-effect mutants by using Bloom-deficient medaka
利用Bloom缺陷青鳉筛选母体效应突变体
- 批准号:
22657055 - 财政年份:2010
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Dorsal determination in vertebrate embryogenesis
脊椎动物胚胎发生的背部决定
- 批准号:
21370103 - 财政年份:2009
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$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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通过细胞因子受体 gp130 介导的信号研究
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11670321 - 财政年份:1999
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$ 2.3万 - 项目类别:
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