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Is arrested embryo development of seedless Kishu mandarin related to genomic imprinting gene?

Is arrested embryo development of seedless Kishu mandarin related to genomic imprinting gene?
无核纪州柑胚胎发育停滞与基因组印记基因有关吗?
批准号:
24658029
负责人:
KITAJIMA Akira
金额:
$2.58万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2014-03-31

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中文摘要
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英文摘要
It is reported that MEA, DME, MET1, FIS2, FWA, PHE, SSRP1 are genomic imprinting genes in Arabidopsis. Citrus homologue genes of these genomic imprinting genes were CLF, SWN, DME, ROS, MET1, FIS2, FWA, FWA2, PHEa, PHEb and SSRP. Genomic DNA and cDNA of Seedless Kishu, Seedy Kishu mandarin and Otachibana pummelo were used for PCR by specific primer of these citrus homologue genes. The amplified products were sequenced. The SNPs were detected in 9 genes of genomic DNA and 8 genes of cDNA. To investigate the genomic imprinting gene expressions in endosperm or embryo crossed by Kishu and Otachibana, different homozygous SNP sequence between Kishu and Otachibana imprinting gene is necessary. These SNP polymorphisms are detected in FWA and PHEb.The expression of FWA in young seeds resulted from Otachibana X Seedless Kishu was investigated, and only 'Seedless Kishu' FWA was expressed. This result indicated that citrus FWA would be genomic imprinting gene.
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Study on molecular mechanisms of fruit abscission and evaluation of parthenocarpy in citrus
  • 批准号:
    22380025
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $12.23万
  • 财政年份:
    2010
  • 负责人:
    KITAJIMA Akira
  • 依托单位:
Study on origin and survey of seedless citrus
  • 批准号:
    19405019
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $9.65万
  • 财政年份:
    2007
  • 负责人:
    KITAJIMA Akira
  • 依托单位:
Study on a mechanism of seedless expression derived from 'Mukaku Kishu' and development of breeding technique in seedless citrus
  • 批准号:
    18380022
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $10.59万
  • 财政年份:
    2006
  • 负责人:
    KITAJIMA Akira
  • 依托单位:
Histological and cytological study of ‘Mukaku Kishu'-derived female sterility in pummelo.
  • 批准号:
    16580020
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $2.3万
  • 财政年份:
    2004
  • 负责人:
    KITAJIMA Akira
  • 依托单位:
海外基金