Study on molecular mechanisms of fruit abscission and evaluation of parthenocarpy in citrus

柑橘果实脱落分子机制研究及单性结实评​​价

• 批准号: 22380025
• 负责人: KITAJIMA Akira
• 金额: $ 12.23万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380025/
• 关键词: 果樹; カンキツ; 単為結果; 生理落果; 果実離脱; マイクロアレイ; 果実; 離脱; 遺伝子発現

The method of detached fruit let incubation on agar medium is useful for induction of fruit abscission to confirm the mechanisms of fruit abscission process during physiological fruit drop in citrus. Abscission layers are rapidly formed in the fruit abscission zone after the induction of abscission and fruit abscission occurs in primary fruitdrop system. However, it is confirmed that no abscission layer is formed in the fruit abscission zone and fruit abscission occurs in secondary fruit drop system. It was evaluatedthat a potential parthenocarpic level is low in ponkan, navel -orange and iyokan, and high in Satsuma mandarin, hyuganatsu and ‘ Hirado’ pummel. It would be possible to evaluate a potential parthenocarpic level by a check of the abscission pattern of detached fruitlet on agar medium during secondary fruit drop. It is supposed that sugar metabolism related genes would be associated to fruit abscission “Decision” process and that β-galactosidase and β-xylosidase would be closely associated to fruit abscission “Performance” process in secondary fruit drop system. It is indicated that an endogenous ethylene is not related to a fruit abscission process and the process of Satsuma mandarin is probably unique.

琼脂培养基上离体培养的方法可用于诱导果实脱落，以明确柑橘生理落果过程中果实脱落过程的机制。诱导脱落后，在落果区迅速形成脱落层，落果发生在初生落果系统中。但经证实，落果区没有形成离层，落果发生在二次落果系统。据评估，椪柑、脐橙和伊羊柑的潜在单性结实水平较低，而萨摩柑橘、hyuganatsu 和‘平户’柚子的潜在单性结实水平较高。通过检查二次落果期间琼脂培养基上分离小果的脱落模式，可以评估潜在的单性结实水平。推测二级落果系统中糖代谢相关基因与果实脱落“决定”过程相关，β-半乳糖苷酶和β-木糖苷酶与果实脱落“表现”过程密切相关。这表明内源乙烯与果实脱落过程无关，且该过程可能是萨摩柑橘独有的。

项目成果

数種カンキツの生理落果期における採取果実の離脱特性

几种柑橘生理落果期采后果实的退果特征

• 发表时间: 2012
• 期刊: 園学研
• 作者: T.Akagi;T.Tsujimoto;A.Ikegami;K.Yonemori;北島 宣 ・李 曦・ 羽生 剛 ・野中勝利・小西 剛・友廣教道・文室政彦・中崎鉄也
• 通讯作者: 北島 宣 ・李 曦・ 羽生 剛 ・野中勝利・小西 剛・友廣教道・文室政彦・中崎鉄也

Evaluations of potential fruit abscission and characteristics of seeded and seedless fruit abscission in several citrus species during physiological fruit drop.

几种柑橘品种生理落果期间潜在果实脱落以及有籽和无籽果实脱落特征的评估。

• 发表时间: 2012
• 作者: Kitajima;A.;X. Li;T. Habu;K. Kataoka;T. Nakazaki and K. Yonrmoti
• 通讯作者: T. Nakazaki and K. Yonrmoti

カンキツにおける二次生理落果の果実離脱に関与する遺伝子の発現解析

柑橘二次落果过程中退果相关基因的表达分析

• 发表时间: 2012
• 期刊: 園学研
• 作者: T.Akagi;A.Ikegami;K.Yonemori;北島 宣・伊勢賢太郎・清水徳朗・羽生剛・李 曦・山崎安津・中崎鉄也
• 通讯作者: 北島 宣・伊勢賢太郎・清水徳朗・羽生剛・李 曦・山崎安津・中崎鉄也

カンキツ果実離脱のトランスクリプトーム解析

柑橘类水果戒断的转录组分析

• 发表时间: 2012
• 作者: 赤木剛士;辻本誠幸;米森敬三;北島宣
• 通讯作者: 北島宣