Fundamental study for citrus chromosome mapping

柑橘染色体定位的基础研究

基本信息

批准号：
08660034
负责人：
KITAJIMA Akira
金额：
$ 1.15万
依托单位：
Kochi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660034/
关键词：
citrus chromosome karyotype snslysis fluorescent stain

项目摘要

Major purpose of this study is to develop new chromosome preparation method for citrus karyotype analysis that is important of gene chromosome mapping, and the second is to try citrus karyotype analysis using this new method and to develop useful chromosome banding method for identification of citrus chromosome.1.Optimum enzymatic treatment was 120 min.incubation of 2% cellulase + 1% pectolyase or 90 min.incubation of 3% cellurase + 2% pectolyase for root tip of in vitro cultured plantlet, and 90min.incubation of 3% cellurase + 1% pectolyase for young leaf tip from orchard tree. The chromosome length of the longest one in each chromosome setreached 4-10mum that was over 2-5 times of the longest chromosome length which had been reported.2.The chromosome numbers of 2n=18 in Poncirus and orange, 2n=20-21 in 'Tosa-Buntan' and 'Suisho-Buntan', 2n=19 in satsuma mandarin, 2n=20 in Fortunella and 2n=19-20 in yuzu were major in this investigation. The karyotyape analysis of Poncirus and orange indicated that both had 2 pairs of metacentric and 7 pairs of submetacentric chromosome, and 6 paires of Poncirus chromosome and 5 pairs of orange chromosome were possible to be identified.3.G banding and N banding method was not available for citrus chromosome. C banding method showed unique banding pattern but repeatability was very low in the present study. CMA/DAPI stain showed stable unique band in 'Tosa-Buntan' chromosome. There were 3 types of chromosome that having 2 bands, 1band and no band.

这项研究的主要目的是开发新的染色体制备方法，以用于柑橘核型分析，这对于基因染色体映射很重要，第二个是使用这种新方法尝试柑橘核型分析，并开发有用的染色体带方法来鉴定柑橘染色体的质子体。用于体外培养植物的根尖和90分钟的3％绩效酶 + 2％果糖酶的含量为3％的3％绩效酶 + 1％果肉酶的幼叶尖端从果园树中进行。染色体最长的染色体长度为4-10mum，超过2-5次，是最长的染色体长度的2-5次。在这项调查中，Yuzu的2n = 19-20是主要的。肺炎和橙色的核叶酸酯分析表明，可以鉴定出2对元中心和7对元中心染色体，以及6对poncirus染色体和5对橙色染色体。3.g3.g clanding和N Banding方法无法用于柠檬色染色体。 C带方法显示出独特的带模式，但在本研究中可重复性非常低。 CMA/DAPI染色在“ Tosa-Buntan”染色体中显示出稳定的独特带。有3种具有2个带，1条带和没有频带的染色体类型。