Development of novel method for searching plant disease resistance gene based on the pathogen perception mechanism

基于病原感知机制寻找植物抗病基因新方法的建立

• 批准号: 24658044
• 负责人: KOBAYASHI Kappei
• 金额: $ 2.58万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658044/
• 关键词: 植物ウイルス; 抵抗性; タンパク質間相互作用; 植物; ウイルス; 抵抗性遺伝子; 酵母ツーハイブリッド法

We tested whether or not it is possible to isolate novel plant disease resistance gene based on the interaction with protein factors known to interact with known resistance protein. Partial cDNA sequences homologous to pepper tobamovirus resistance gene, L, from an eggplant cultivar were screened for the interaction with LIPM1 and LIPM2, both of which were identified as interaction partners of pepper L resistance protein. The results suggest that the both LIPM1 and LIPM2 interact with diverse L-homolog sequences from eggplant and that they are unlikely to be involved in the determination of pathogen specificity of resistance protein. Gene-silencing study suggest that LIPM2 has a role in the regulation of cell-death signaling.

我们测试了是否有可能基于与已知抗性蛋白相互作用的已知蛋白因子的相互作用分离新的植物抗病基因。从茄子品种中筛选辣椒多巴病毒抗性基因L的部分同源cDNA序列，并与辣椒L抗性蛋白的互作伙伴LIPM1和LIPM2进行互作。结果表明，LIPM1和LIPM2与茄子中多种l -同源序列相互作用，不太可能参与抗性蛋白病原菌特异性的测定。基因沉默研究提示LIPM2参与细胞死亡信号的调控。

项目成果

Gentian Kobu-sho-associated virus: a tentative, novel double-stranded RNA virus that is relevant to gentian Kobu-sho syndrome

• DOI: 10.1007/s10327-012-0423-5
• 发表时间: 2013-01-01
• 期刊: JOURNAL OF GENERAL PLANT PATHOLOGY
• 影响因子: 1.2
• 作者: Kobayashi, Kappei;Atsumi, Go;Sekine, Ken-Taro
• 通讯作者: Sekine, Ken-Taro

Expression of Cauliflower mosaic virus Transactivator/viroplasmin in transgenic tobacco under the control of dexamethasone-inducible promoter

地塞米松诱导型启动子控制下花椰菜花叶病毒反式激活子/病毒纤溶酶在转基因烟草中的表达

• 发表时间: 2013
• 作者: Alam;M. M.;Nakamura;N.;Ichikawa;H.;Kobayashi;K.;Yaeno;T.;Yamaoka;N. and Nishiguchi;M.;菅井維之・新崎裕樹・八丈野孝・小林括平・西口正通・山岡直人;香口智宏・菅井維之・中下香・久野裕・小林括平・西口正通・山岡直人・八丈野孝;Waliullah Sumyya
• 通讯作者: Waliullah Sumyya

Functional differentiation in the LRR domains of closely related plant virus resistance proteins that recognize common Avr proteins.

识别常见 Avr 蛋白的密切相关植物病毒抗性蛋白的 LRR 结构域的功能分化。

• 发表时间: 2012
• 期刊: Molecular Plant-Microbe Interactions
• 影响因子: 3.5
• 作者: Ken-Taro Sekine;Reiko Tomita;Shigeharu Takeuchi;Go Atsumi;Hiromasa Saitoh;Hiroyuki Mizumoto;Akinori Kiba;Naoto Yamaoka;Masamichi Nishiguchi;Yasufumi Hikichi and Kappei Kobayashi.
• 通讯作者: Yasufumi Hikichi and Kappei Kobayashi.

Identification and characterization of Lisianthus necrotic ringspot virus, a novel distinct tospovirus species causing necrotic disease of lisianthus (Eustoma grandiflorum)

• DOI: 10.1007/s10327-014-0503-9
• 发表时间: 2014-01
• 期刊: Journal of General Plant Pathology
• 影响因子: 1.2
• 作者: Y. Shimomoto;K. Kobayashi;M. Okuda

Evaluation of the durability of N´ resistance gene to Pepper mild mottle virus using random mutagenesis of coat protein genes

使用外壳蛋白基因的随机诱变评估 N´ 抗性基因对胡椒轻度斑驳病毒的耐久性

• 发表时间: 2012
• 作者: Sekine K.-T.;Tomita R.;Atsumi G.;Chen H.;Kaido M.;Yamaoka N.;Nishiguchi M.;Okuno T. and Kobayashi K.;出原健吾
• 通讯作者: 出原健吾