Novel strategy to awake gene cluster using small molecules that activate LAL family transcriptional regulator

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Genome sequencing of Streptomyces spp. revealed many unknown secondary metabolite gene clusters that might be potential sources for a medicinal drug and an agricultural chemical. The objective of the study is an activation of secondary metabolite gene clusters using small molecules. LAL family transcriptional regulators are widely found in Streptomyces spp. Among them, we focused on RevU which exists in reveromycin biosynthetic gene cluster. We searched a small molecule which activates reveromycin production, and optimized its chemical structure. This study gave great insights for the mechanism of the activation of secondary metabolite gene cluster.