Experimental embryological study to regenerate individual from dissociated blastomeres in goldfish.

从金鱼分离的卵裂球中再生个体的实验胚胎学研究。

• 批准号: 24658163
• 负责人: YAMAHA ETSURO
• 金额: $ 2.58万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658163/
• 关键词: 発生工学; 胚様体; スフェロイド; キンギョ; 硬骨魚類; 中胚葉; 卵黄細胞; ゼブラフィッシュ; 深層細胞; 増殖因子; 多分化能

It is reported that blastomeres of early fish embryo have pluripotency during blastula satage. The properties of dissociated blastomeres were analyzed to develop new techniques that indivicuals regenrate from those cells.Dissociated blastomeres lost their pluripotency when transplanted to host blastula after longer cultivation under dissociated condition in which germ-cell line was maintained. Additional embryonic axis was induced when dissociated blastomeres treated with some growth factors were transplanted into host blastula. This results suggest that blastomeres were artificially destined their developmental fate. Dissociated blastomeres formed spheroid aggregates when cultured in vitro on the extenrla adhesive matrix in the simple Ringer's solution. But embryoid was not induced from this spheroid aggregate transplanted on the yolk cell, yet.

据报道，早期鱼类胚胎的卵裂球在囊胚发育过程中具有多能性。对分离的卵裂球的特性进行了分析，以开发从这些细胞中再生个体的新技术。在保持生殖细胞系的分离条件下，分离的卵裂球在长期培养后移植到宿主囊胚中失去了多能性。将经一定生长因子处理的解离的卵裂球移植到宿主囊胚中，可诱导出额外的胚轴。这一结果表明，卵裂球是人为地决定了它们的发育命运。分离的卵裂球在体外培养于简单林格氏溶液中的延长型黏附基质上，可形成球状聚集体。但是，移植到卵黄细胞上的这种球形聚集体还没有诱导出胚状体。