Experimental embryological study to regenerate individual from dissociated blastomeres in goldfish.

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It is reported that blastomeres of early fish embryo have pluripotency during blastula satage. The properties of dissociated blastomeres were analyzed to develop new techniques that indivicuals regenrate from those cells.Dissociated blastomeres lost their pluripotency when transplanted to host blastula after longer cultivation under dissociated condition in which germ-cell line was maintained. Additional embryonic axis was induced when dissociated blastomeres treated with some growth factors were transplanted into host blastula. This results suggest that blastomeres were artificially destined their developmental fate. Dissociated blastomeres formed spheroid aggregates when cultured in vitro on the extenrla adhesive matrix in the simple Ringer's solution. But embryoid was not induced from this spheroid aggregate transplanted on the yolk cell, yet.