Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy.
Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy.
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DOI：
10.1371/journal.pone.0035227
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kawazu S
中科院分区：
文献类型：
--
作者：
Asada M;Goto Y;Yahata K;Yokoyama N;Kawai S;Inoue N;Kaneko O;Kawazu S
牛巴贝虫是一种顶端复门红细胞内原生动物寄生虫，通过蜱传播后在牛中诱发巴贝虫病。在顶复门寄生虫生命周期的特定阶段，例如恶性疟原虫的子孢子和弓形虫的速殖子，宿主细胞通过一种独特的、活跃的过程（称为“滑行运动”）进行入侵。然而，目前尚未完全了解牛伯氏杆菌的裂殖子如何靶向并侵入宿主红细胞（RBC），并且迄今为止尚未在寄生虫中观察到滑行运动。通过延时视频显微镜揭示了牛芽孢杆菌裂殖子的滑动运动。记录的图像显示，该过程包括裂殖子从受感染的红细胞中排出、滑行运动以及随后侵入新的红细胞。牛芽孢杆菌裂殖子的滑动运动与弓形虫速殖子的螺旋滑动相似。使用针对牛芽孢杆菌裂殖子表面抗原1的抗血清，通过间接免疫荧光测定法检测裂殖子留下的痕迹。肌动蛋白丝聚合或解聚对滑行运动的抑制表明滑行运动是由肌动球蛋白依赖性过程驱动的。此外，我们还揭示了寄生液泡破裂的时间。对膜染色牛红细胞的延时图像分析显示，寄生液泡在入侵后十分钟内形成和破裂。这是关于牛双歧杆菌滑行运动的第一份报告。由于疟原虫的裂殖子不会在基质上滑动，因此牛芽胞杆菌裂殖子的滑动运动是一个值得注意的发现。
Babesia bovis is an apicomplexan intraerythrocytic protozoan parasite that induces babesiosis in cattle after transmission by ticks. During specific stages of the apicomplexan parasite lifecycle, such as the sporozoites of Plasmodium falciparum and tachyzoites of Toxoplasma gondii, host cells are targeted for invasion using a unique, active process termed “gliding motility”. However, it is not thoroughly understood how the merozoites of B. bovis target and invade host red blood cells (RBCs), and gliding motility has so far not been observed in the parasite. Gliding motility of B. bovis merozoites was revealed by time-lapse video microscopy. The recorded images revealed that the process included egress of the merozoites from the infected RBC, gliding motility, and subsequent invasion into new RBCs. The gliding motility of B. bovis merozoites was similar to the helical gliding of Toxoplasma tachyzoites. The trails left by the merozoites were detected by indirect immunofluorescence assay using antiserum against B. bovis merozoite surface antigen 1. Inhibition of gliding motility by actin filament polymerization or depolymerization indicated that the gliding motility was driven by actomyosin dependent process. In addition, we revealed the timing of breakdown of the parasitophorous vacuole. Time-lapse image analysis of membrane-stained bovine RBCs showed formation and breakdown of the parasitophorous vacuole within ten minutes of invasion. This is the first report of the gliding motility of B. bovis. Since merozoites of Plasmodium parasites do not glide on a substrate, the gliding motility of B. bovis merozoites is a notable finding.
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影响因子：
4.4
作者：
Chauvin A;Moreau E;Bonnet S;Plantard O;Malandrin L
通讯作者：
Malandrin L
影响因子：
3.3
作者：
Håkansson, S;Morisaki, H;Sibley, LD
通讯作者：
Sibley, LD
影响因子：
4.8
作者：
Wickham, ME;Culvenor, JG;Cowman, AF
通讯作者：
Cowman, AF
影响因子：
1.5
作者：
Siden-Kiamos, Inga;Pinder, Jennifer C.;Louis, Christos
通讯作者：
Louis, Christos
DOI：
10.1111/j.1550-7408.1978.tb04167.x
发表时间：
1978-01-01
期刊：
JOURNAL OF PROTOZOOLOGY
影响因子：
--
作者：
LANGRETH, SG;JENSEN, JB;TRAGER, W
通讯作者：
TRAGER, W