Promotion of development of in vitro produced bovine embryos by using emulsified lipophilic nutrients

我们开发了一种简单稳定的方法来乳化具有生物活性的亲脂性营养物质，并将其添加到体外培养的牛胚胎培养基中。α -生育酚和β -胡萝卜素的乳化混合物（emVEBC）提高了囊胚的孵化率，而乳化油酸则降低了囊胚的发育。为了阐明emVEBC对胚胎发育有利作用的机制，我们对囊胚进行了基于微阵列的转录组学分析，并列出了候选的负责基因。

We developed a simple and stable method for emulsification of bioactive lipophilic nutrients intended to add them into culture media of in vitro produced bovine embryos. Emulsified mixture of alpha-tocopherol and beta-carotene (emVEBC) enhanced hatching rate of blastocysts, whereas emulsified oleic acid decreased blastocyst development. To elucidate mechanisms of the favorable effects of emVEBC on embryonic development, a microarray-based transcriptomic analysis of blastocysts was implemented and candidates of responsible genes were listed.