Promotion of development of in vitro produced bovine embryos by using emulsified lipophilic nutrients

利用乳化亲脂性营养物质促进体外牛胚胎发育

• 批准号: 24658233
• 负责人: IKEDA Shuntaro
• 金额: $ 2.58万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658233/
• 关键词: ウシ; 受精卵; 初期胚; 体外受精; 脂質; 乳化; 体外培養; IVM-F-C; 胚盤胞; 栄養と繁殖

We developed a simple and stable method for emulsification of bioactive lipophilic nutrients intended to add them into culture media of in vitro produced bovine embryos. Emulsified mixture of alpha-tocopherol and beta-carotene (emVEBC) enhanced hatching rate of blastocysts, whereas emulsified oleic acid decreased blastocyst development. To elucidate mechanisms of the favorable effects of emVEBC on embryonic development, a microarray-based transcriptomic analysis of blastocysts was implemented and candidates of responsible genes were listed.

我们开发了一种简单而稳定的乳化生物活性亲脂性营养素的方法，旨在将它们添加到体外生产的牛胚胎的培养基中。α-生育酚和β-胡萝卜素的乳化混合物（emVEBC）提高囊胚孵化率，而乳化油酸降低囊胚发育。为了阐明emVEBC对胚胎发育有利影响的机制，实施了基于微阵列的囊胚转录组学分析，并列出了负责基因的候选者。

项目成果

Simple method for emulsification of lipophilic nutrients that affect preimplantation development of bovine embryos in vitro

乳化影响牛胚胎植入前体外发育的亲脂性营养物质的简单方法

• DOI: 10.1071/rdv26n1ab86
• 发表时间: 2014
• 期刊: Reprod. Fertil. Dev
• 作者: Asada;M.;Goto;Y.;Yahata;K.;Yokoyama;N.;Kawai;S.;Inoue;N.;Kaneko;O.;and Kawazu;S.-I;Ikeda S
• 通讯作者: Ikeda S