The roles of methylation pathway in epigenetics of bovine preimplantation embryos

我们证明(1)牛卵母细胞和着床前胚胎表达甲基化途径酶；(2)在甲基化途径的代谢物中，同型半胱氨酸影响DNA甲基化水平，蛋氨酸通过转化为s -腺苷蛋氨酸参与了牛着床前发育过程中桑葚胚向囊胚的转变。甲基化途径在牛着床前发育中的存在及其重要性。

We demonstrated that (1) bovine oocytes and preimplantation embryos express methylation pathway enzymes ; (2) among the metabolites in methylation pathway, homocysteine affects the level of DNA methylation, and methionine, thorough its conversion to S-adenosylmethionine, is involved in morula-to-blastocyst transition during bovine preimplantation development. The existence and importance of methylation pathway in bovine preimplantation development were suggested.