Study on physiological role for novel peptide, NU1 and NU2

侧脑室注射新型多肽NU1和NU2可剂量依赖性地增加大鼠的摄食量和体温。用基因末端分析法检测侧脑室注射这些多肽后下丘脑和中脑/脑干的mRNA表达水平，发现前列腺素E合成酶和环氧合酶的mRNA表达显著增加。而预先给予消炎痛则完全阻断了NU1和NU2引起的体温升高。这些结果表明，NU1和NU2通过前列腺素E升高体温。

Intracerebroventricular (icv) injection of novel peptide, NU1 and NU2, increased the food intake and body temperature in a dose-dependent manner in rats. When expression of mRNA levels in the hypothalamus and midbrain/brainstem after icv injection of these peptides were examined using gene tip analysis, prostaglandin E synthetase mRNA and cyclooxygenase mRNA expressions increased dramatically. On the other hand, pretreatment with indomethacin completely blocked the increase of body temperature induced by NU1 and NU2. These results suggest that NU1 and NU2 increase the body temperature through prostaglandin E.