Analysis of mechanism of hierarchical rhythm and biological dock in mammals and avian species

哺乳动物和鸟类的等级节律和生物停靠机制分析

• 批准号: 13460134
• 负责人: MURAKAMI Noboru
• 金额: $ 8.19万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460134/
• 关键词: Biological rhythm; suprachiasmatic nucleus; Circadian rhythm; neuromedine U; orphan receptor; 生体リズム; 位相変位; 反射; オーファンレセプター; 視交叉土核; 生物時計

By immunohistochemical analysis, we detected a gut-brain peptide, neuromedin U (NMU), in the suprachiasmatic nucleus (SCN) which is the site of master circadian oscillator. The NMU mRNA expressed circadian rhythm with the peak at CT4-8 in the SCN. The two NMU-binding receptors (NMU-R1 and NMU-R2) were also expressed in the SCN, but phase angles were different between both receptors. Intracerebroventricular injection (ICV) of NMU induced Fos protein in the SCN cells and caused a phase dependent phase shift of the circadian locomotor activity rhythm. The magnitude of phase shift was dose-dependent. This NMU-induced phase-shift was non-photic type. RT-PCR analyses revealed increases in the expression of immediate early genes, such as c-fos, NGFI-A, NGFI-B and JunB in SCN. Furthermore, ICV injection of NMU increased the expression of Per1, but not Per2 in the SCN. These results indicate that NMU may play some important role for circadian oscillator by autocrine or paracrine action in SCN.

通过免疫组织化学分析，我们发现了一种肠脑肽，神经介肽U（NMU），在视交叉上核（SCN），这是主昼夜振荡器的网站。NMU mRNA在SCN表达呈昼夜节律，峰值在CT 4 -8。两种NMU结合受体（NMU-R1和NMU-R2）也在SCN中表达，但两种受体之间的相位角不同。侧脑室注射NMU可诱导SCN细胞内Fos蛋白的表达，并引起昼夜运动活动节律的时相依赖性相移。相移的幅度具有剂量依赖性。这种由NMU引起的相移是非光性的。RT-PCR分析显示，SCN中的立即早期基因，如c-fos，NGFI-A，NGFI-B和JunB的表达增加。此外，ICV注射NMU增加了SCN中Per 1的表达，但不增加Per 2的表达。这些结果表明，NMU可能通过自分泌或旁分泌作用在SCN的昼夜节律振荡中发挥重要作用。

项目成果

K.Toshinai: "Ghrelin-induced food intake is mediated via the orexin pathway."Endocrinology. 144(4). 1506-1512 (2003)

K.Toshinai：“生长素释放肽诱导的食物摄入是通过食欲素途径介导的。”内分泌学。

K Nakahara: "Intracellular Ca^<2+> signaling pathway is in volved in light-induced phase advance, cell. but may not be in phase delay, of o'rcadian melatonin rhythm in chick pineal"Journal of Pineal Research. 30. 234-242 (2001)

K Nakahara：“细胞内Ca^2信号通路参与光诱导的细胞相位提前，但可能不参与小鸡松果体褪黑激素节律的相位延迟”Journal of Pineal Research。

T.Hayashida: "Ghrelin in neonatal rats: distribution in stomach and its possible role"J. Endocrinology. 173. 239-245 (2002)

T.Hayashida：“新生大鼠中的 Ghrelin：胃中的分布及其可能的作用”J.

Nakahara K.: "Pituitary adenylate cyclase-activating polypeptide (PACAP) is involved in melatonin release via the specific receptor PACAP-γ, but not in…"Brain Research. (2002)

Nakahara K.：“垂体腺苷酸环化酶激活多肽 (PACAP) 通过特定受体 PACAP-γ 参与褪黑激素的释放，但不参与……”Brain Research (2002)。

Toshinai K: "Ghrelin-induced food intake is mediated via the orexin pathway."Endocrinology. 144. 1506-1512 (2003)

Toshinai K：“生长素释放肽诱导的食物摄入是通过食欲素途径介导的。”内分泌学。