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Can soil hardness alleviate aluminium stress in plant?

土壤硬度可以缓解植物的铝胁迫吗？

基本信息

批准号：
24658274
负责人：
YANO Katsuya
金额：
$ 2.58万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2012
资助国家：
日本
起止时间：
2012 至无数据
项目状态：
已结题

项目摘要

This study was aimed to know 1) physical stimuli to root tips can increase production of root-tip mucilage, 2) such an increase can reduce Al-accumulation in the root tips, and consequently 3) stress impact of Al can be alleviated. To create the presence or absence of the stimuli to roots, maize (Zea mays L.) plants were hydroponically grown with or without sand, respectively. Plants grown with sand had numerous root tips with mucilage besides its volume per tip was also larger comparing to ones without sand, indicating that the presence of the stimuli can promote mucilage production. Giving AlCl3at various concentrations, Al injury could occur only at 10 Μm without sand but was not fully induced even at 1000 Μm with sand, revealing that Al toxicity is alleviated in the presence of the root stimuli. Root tips collected from conditions in the presence or absence of sand were divided into ones with or without mucilage, and then exposed to AlCl3 solution at 1000 Μm. The tips with mucilage showed lower Al -accumulation than ones without it, suggesting that mucilage could protect the tip against Al. On the other hand, there was no significant difference in Al-accumulation between the tips with mucilage and ones once removed it. To seek the reason for this, the tips once removed mucilage were monitored and found that visible mucilage could recover within 5 minutes. We have assumed that such a rapid recovery of mucilage is responsible for no significant effect of mucilage removal.

本研究的目的是了解：1）对根尖的物理刺激可以增加根尖粘液的产生，2）这种增加可以减少根尖中铝的积累，从而3）可以减轻铝的胁迫影响。为了产生对根部刺激的存在或不存在，玉米（Zea mays L.）植物分别在有或没有沙子的情况下进行水培生长。用沙子生长的植物有许多带有粘液的根尖，而且每个根尖的体积也比没有沙子的植物大，这表明刺激的存在可以促进粘液的产生。给予不同浓度的AlCl3，仅在没有沙子的10μm处才可能发生铝损伤，但即使在有沙子的1000μm处也不能完全诱导，这表明在根部刺激存在的情况下铝毒性减轻。将在存在或不存在沙子的条件下收集的根尖分为有粘液或无粘液的根尖，然后暴露于1000μm的AlCl 3 溶液中。有粘液的尖端显示出比没有粘液的尖端更低的铝积累，这表明粘液可以保护尖端免受铝的侵害。另一方面，有粘液的尖端和去除粘液的尖端之间的铝积累没有显着差异。为了找出原因，对去除粘液后的吸头进行监测，发现可见的粘液可以在 5 分钟内恢复。我们假设粘液的如此快速的恢复是导致粘液去除没有显着效果的原因。