Development of the selective capture molecule for 8-nitroguanosine

在这项研究中，我们通过引入不同长度的硫代烷基脲连接单元(NitroG-GRGRAP)，设计了新的含硫醇的识别分子，用于8-硝基鸟苷的共价捕获。带有C3连接基的硝基G-GRAP化合物即使在低浓度下也表现出最有效的鸟苷化，支持选择性形成络合物以有效地反应8-硝基-G。将环烯单元连接到G-钳分子上，识别8-oxoGTP，作为环烯-硝基G-GRAP分子的模型化合物。在水溶液中，Cyclen-oxoG-钳的锌络合物能够区分8-oxoGTP和GTP。在硝基G-GRAPH分子中引入环化单元，使8-硝基GMP不与锌离子形成络合物而发生阳离子反应。综上所述，本研究为8-硝基GTP和8-硝基GMP共价捕获奠定了分子基础。

In this study, we designed new thiol-containing recognition molecules for the covalent capture of 8-nitroguanosine based on the Gclamp skeleton by introducing thioalkylurea linker units of varying length (nitroG-grasp). The nitroG-grasp compound with C3 linker exhibited the most efficient guanylation even at low concentrations, supporting the selective complex formation for efficient reactivity for 8-nitro-G. The cyclen unit was attached to G-clamp molecule to recognize 8-oxoGTP, as a model compound of the cyclen-nitroG-grasp molecule. The Zn-complex of the cyclen-oxoG-clamp was able to discriminate 8-oxoGTP from GTP in aqueous media. The cyclen unit was also introduced to the nitroG-grasp molecule, which exhibited guanylation reaction for 8-nitroGMP without forming complex with zinc cation. In conclusion, this study has established the molecular basis for covalent capture of 8-nitroGTP and 8-nitroGMP.