Development of the selective capture molecule for 8-nitroguanosine

在这项研究中，我们设计了新的含硫醇的识别分子，用于在Gclamp骨架的基础上，通过引入不同长度的硫代烷基脲连接单元（氮抓握）来共价捕获8-硝基鸟苷。含C3连接剂的抓氮化合物即使在低浓度下也表现出最有效的胍基化反应，支持8-硝基g的选择性络合物形成。将cyclen单元附着在G-clamp分子上识别8-oxoGTP，作为cyclen- nitrogen -grasp分子的模型化合物。cyclen-oxoG-clamp的zn配合物能够在水介质中区分8-oxoGTP和GTP。将循环单元引入到抓氮分子中，8-nitroGMP发生胍基化反应而不与锌阳离子形成配合物。总之，本研究建立了8-nitroGTP和8-nitroGMP共价捕获的分子基础。

In this study, we designed new thiol-containing recognition molecules for the covalent capture of 8-nitroguanosine based on the Gclamp skeleton by introducing thioalkylurea linker units of varying length (nitroG-grasp). The nitroG-grasp compound with C3 linker exhibited the most efficient guanylation even at low concentrations, supporting the selective complex formation for efficient reactivity for 8-nitro-G. The cyclen unit was attached to G-clamp molecule to recognize 8-oxoGTP, as a model compound of the cyclen-nitroG-grasp molecule. The Zn-complex of the cyclen-oxoG-clamp was able to discriminate 8-oxoGTP from GTP in aqueous media. The cyclen unit was also introduced to the nitroG-grasp molecule, which exhibited guanylation reaction for 8-nitroGMP without forming complex with zinc cation. In conclusion, this study has established the molecular basis for covalent capture of 8-nitroGTP and 8-nitroGMP.