Artificial induction of DNA methylation

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We planned to establish two novel strategies to introduce artificial DNA methylation using zinc finger fusion proteins and small RNAs. One is the introduction of de novo DNA methylation in embryonic male germ cells by using the fusion protein of zinc finger protein and MIWI2 protein. The other is the introduction of DNA methylation in culture cells by using the triplex nucleic acids consisting of two DNA strands and one RNA strand. Although, unfortunately, the latter experiment did not work, we succeeded in establishing the latter one. Following the success, we are currently analyzing the function of MIWI2 in the embryonic male germ cells.