Searching for the factors concerning hematopoiesis

寻找与造血相关的因素

基本信息

  • 批准号:
    10557012
  • 负责人:
  • 金额:
    $ 6.72万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1998
  • 资助国家:
    日本
  • 起止时间:
    1998 至 1999
  • 项目状态:
    已结题

项目摘要

One of the purposes of this study is cloning the genes of the factors that have some roles in early hematopoiesis. For this purpose, we combined the in vitro hematopoietic differentiation induction method from mouse embryonic stem cells and the signal sequence trap method that is a method to select secretory proteins containing signal peptide. cDNA library was constructed from the day 5 induced mesodermal cell colonies in which hemangioblasts and hematopoietic progenitors existed. As a result, cDNA named ESOP-1 was cloned. The deduced amino acid sequence shows 160 amino acids protein. Human ESOP-1 was also cloned and shows 64% homology. ESOP-1 cDNA was highly expressed in embryos at day 7.5 of gestation. This gene was also expressed in both adult and embryonic hematopoietic system. Antibody was produced against this protein and immuno staining revealed that the protein was expressed in yolk sac, developing nervous system and adult genital organs.The other outcome is the analysis of the … More fuction of Bcl-X gene on erythropoiesis. We utilized Bcl-X null mouse embryonic stem cells (ES cells), and showed that Bcl-X is indispensable for the production of both embryonic primitive erythrocytes (EryP) and adult definitive erythrocytes (EryD) at the end of their maturation. In vivo, bcl-x null ES cells did not contribute to circulating EryD in adult chimeric mice that were produced by blastocyst microinjection of the bcl-x null ES cells. bcl-x null EryP and EryD were produced by in vitro differentiation induction of ES cells on a macrophage colony-stimulating factor deficient stromal cell line OP9, and further analysis was carried out. The emergence of immature EryP and EryD from bcl-x null ES cells was similar to that from normal ES cells. However, prominent cell death of bcl-x null EryP and EryD occurred when the cells matured. Erythropoietin is thought to prevent the apoptotic cell death of erythroid progenitor cells, while the anti-apoptotic function of bcl-x acts at the very end of maturation. Less
本研究的目的之一是克隆早期造血相关因子的基因。为此,我们将小鼠胚胎干细胞的体外造血分化诱导方法和信号序列捕获方法(一种选择含有信号肽的分泌蛋白的方法)相结合。从第5天诱导的中胚层细胞集落中构建cDNA文库,其中存在成血管细胞和造血祖细胞。结果,克隆了命名为ESOP-1的cDNA。推导的氨基酸序列为160个氨基酸。人ESOP-1也被克隆,并显示64%的同源性。ESOP-1 cDNA在妊娠7.5天的胚胎中高度表达。该基因在成人和胚胎造血系统中均有表达。免疫组化结果表明,该蛋白在卵黄囊、发育中的神经系统和成体生殖器官中均有表达,并与正常胚胎的表达进行了比较。 ...更多信息 Bcl-X基因在红细胞生成中作用我们利用Bcl-X无效的小鼠胚胎干细胞(ES细胞),并表明,Bcl-X是必不可少的生产胚胎原始红细胞(EryP)和成人定形红细胞(EryD)在其成熟结束。在体内,bcl-x空ES细胞并没有贡献循环EryD在成年嵌合小鼠,所产生的囊胚显微注射的bcl-x空ES细胞。通过在巨噬细胞集落刺激因子缺陷基质细胞系OP 9上体外分化诱导ES细胞产生bcl-x null EryP和EryD,并进行进一步分析。未成熟的EryP和EryD从bcl-x缺失的ES细胞的出现类似于从正常ES细胞。然而,当细胞成熟时,bcl-x null EryP和EryD发生显著的细胞死亡。促红细胞生成素被认为可以防止红系祖细胞的凋亡性细胞死亡,而bcl-x的抗凋亡功能作用于成熟的最后阶段。少

项目成果

期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
T. Kimura, K. Yomogida, N. Iwai, Y. Kato, T. Nakano: "Molecular cloning and genomic organization of mouse homologue of Drosophila germ cell-less and its expression in germ lineage cells"Biochem Biophys Res Commun. 262. 223-30 (1999)
T. Kimura、K. Yomogida、N. Iwai、Y. Kato、T. Nakano:“果蝇无生殖细胞小鼠同源物的分子克隆和基因组组织及其在生殖谱系细胞中的表达”Biochem Biophys Res Commun。
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    0
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  • 通讯作者:
T. Takahashi, P. Dai, S. Ishii, T. Nakano, et al.: "Inhibitory interaction of c-Myb and GATA-1 via transcriptional co-activator CBP"Oncogene. 19. 134-140 (2000)
T. Takahashi、P. Dai、S. Ishii、T. Nakano 等人:“通过转录共激活因子 CBP 抑制 c-Myb 和 GATA-1 的相互作用”癌基因。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
仲野 徹: "岩波講座・現代医学の基礎8 : 免疫と血液の科学、第一章、血液細胞の発生と分化"岩波書店. 19 (1999)
中野彻:“岩波讲座:现代医学基础 8:免疫与血液科学,第一章,血细胞的发育和分化”岩波书店 19 (1999)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
K. Kato, T. Nakano, K. Tashiro, T. Honjo, et al.: "ESOP-1, a secreted protein expressed in the hematopoietic, nervous and reproductive systems of embryonic and adult mouse"Blood. (in press). (2000)
K. Kato、T. Nakano、K. Tashiro、T. Honjo 等人:“ESOP-1,一种在胚胎和成年小鼠的造血、神经和生殖系统中表达的分泌蛋白”血液。
  • DOI:
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  • 期刊:
  • 影响因子:
    0
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NAKANO Toru其他文献

NAKANO Toru的其他文献

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{{ truncateString('NAKANO Toru', 18)}}的其他基金

Artificial induction of DNA methylation
人工诱导DNA甲基化
  • 批准号:
    24659135
  • 财政年份:
    2012
  • 资助金额:
    $ 6.72万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
A study of Chinese lianhuanhua(連環画)in 1950's
20世纪50年代中国连环花研究
  • 批准号:
    23820074
  • 财政年份:
    2011
  • 资助金额:
    $ 6.72万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
Epigenetic Regulation in Development and Differentiation
发育和分化中的表观遗传调控
  • 批准号:
    21249016
  • 财政年份:
    2009
  • 资助金额:
    $ 6.72万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Molecular mechanisms of the maintenance of stem cell systems
干细胞系统维持的分子机制
  • 批准号:
    18390088
  • 财政年份:
    2006
  • 资助金额:
    $ 6.72万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Function of transcription factors in hematopoietic differentiation
转录因子在造血分化中的作用
  • 批准号:
    16390277
  • 财政年份:
    2004
  • 资助金额:
    $ 6.72万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
MOLECULAR BASIS OF STEM CELL SYSTEMS AND ITS APPLICATION
干细胞系统的分子基础及其应用
  • 批准号:
    14207006
  • 财政年份:
    2002
  • 资助金额:
    $ 6.72万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Manipulation of hematopoietic cells for regenerative medicine
再生医学中造血细胞的操作
  • 批准号:
    12557080
  • 财政年份:
    2000
  • 资助金额:
    $ 6.72万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular Mechanisms for Maintaining Stem Cell Immaturity
维持干细胞不成熟的分子机制
  • 批准号:
    12470026
  • 财政年份:
    2000
  • 资助金额:
    $ 6.72万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Fundamental molecular mechanisms of stem cell system
干细胞系统的基本分子机制
  • 批准号:
    10470059
  • 财政年份:
    1998
  • 资助金额:
    $ 6.72万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Cloning and analysis of the genes during the induction of mouse embryongenesis
小鼠胚胎发生诱导过程中基因的克隆与分析
  • 批准号:
    08457037
  • 财政年份:
    1996
  • 资助金额:
    $ 6.72万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似国自然基金

基于油菜裂外壁小孢子胚胎发生系统探讨胚胎发育早期极性的建立、分裂模式及细胞命运抉择
  • 批准号:
    30970279
  • 批准年份:
    2009
  • 资助金额:
    28.0 万元
  • 项目类别:
    面上项目

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剖析胚胎发生过程中的核糖体暂停:从整体和单分子研究到整个胚胎表型
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胚胎发生过程中基因表达的时序机制。
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