Gene expression analysis of metastatic breast cancer in lymph nodes using residual OSNA sample

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The one-step nucleic acid amplification (OSNA) assay is a rapid and reliable procedure for the detection of sentinel lymph node (SLN) metastases using molecular biological techniques during the operation of breast cancer. In the present study, we focused “maspin”, which is the poor prognostic factor of human breast cancer and its expression was not detected in normal lymph nodes. The used samples were OSNA;0 (5 samples), OSNA;1+ (34 samples) and OSNA;3+ (59 samples). After the extraction of total RNA from residual OSNA samples, cDNA was generated and quantitative Real-time RT-PCR analysis was performed using human maspin primer/probe set. Maspin mRNA expression was noted in 46 samples (49.5%) and was significantly frequent in OSNA 2+ (p