Selective isolation of pseudopodia by using excimer laser ablation

使用准分子激光烧蚀选择性分离伪足

• 批准号: 24659184
• 负责人: INOUE Takao
• 金额: $ 2.5万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659184/
• 关键词: 癌の浸潤・転移; プロテオミクス

Pseudopodia (actin-rich protrusion) are considered to be important for cancer metastasis. Tumor cells were cultured on a porous membrane to form pseudopodia in membrane pores. An excimer laser, which was used for LASIK, was employed for removing cell body on a membrane to selectively isolate pseudopodia in membrane pores. The proteins extracted from pseudopodia were analyzed and identified. Of those proteins, alpha-Parvin, which highly expressed in pseudopodia, was characterized. The results showed that the protein was involved in pseudopodial length and density, and cell migration ability.

假足（富含肌动蛋白的突起）被认为对癌症转移很重要。将肿瘤细胞培养在多孔膜上，形成膜孔中的假足病。用于LASIK的准分子激光器用于去除膜上的细胞体，以选择性地分离膜孔中的假卵形。分析并鉴定出从假虫病中提取的蛋白质。在这些蛋白质中，在伪足中高度表达的α-帕尔文被表征了。结果表明，该蛋白质参与伪的长度和密度以及细胞迁移能力。

项目成果

Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve-mast cell interaction in a hapten-induced mouse model of atopic dermatitis

• DOI: 10.1111/bjd.12108
• 发表时间: 2013-04-01
• 期刊: BRITISH JOURNAL OF DERMATOLOGY
• 影响因子: 10.3
• 作者: Hagiyama, M.;Inoue, T.;Ito, A.
• 通讯作者: Ito, A.

新規骨芽細胞接着分子cell adhesion molecule 1は骨芽細胞分化過程において一過性に発現する

一种新型的成骨细胞粘附分子，即细胞粘附分子1，在成骨细胞分化过程中瞬时表达。

• 发表时间: 2012
• 作者: 井上敬夫;萩山満;榎木英介;伊藤彰彦
• 通讯作者: 伊藤彰彦