Selective isolation of pseudopodia by using excimer laser ablation

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Pseudopodia (actin-rich protrusion) are considered to be important for cancer metastasis. Tumor cells were cultured on a porous membrane to form pseudopodia in membrane pores. An excimer laser, which was used for LASIK, was employed for removing cell body on a membrane to selectively isolate pseudopodia in membrane pores. The proteins extracted from pseudopodia were analyzed and identified. Of those proteins, alpha-Parvin, which highly expressed in pseudopodia, was characterized. The results showed that the protein was involved in pseudopodial length and density, and cell migration ability.