Screening of novel anti-citrullinated protein antibodies for rheumatoid arrhtiris by protein array

蛋白芯片筛选新型抗类风湿性关节炎的瓜氨酸蛋白抗体

• 批准号: 24659274
• 负责人: OHMURA Koichiro
• 金额: $ 2.5万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659274/
• 关键词: 免疫血清学; 抗CCP抗体; ACPA; 関節リウマチ; バイオマーカー; 抗CCP抗体陰性RA; AlphaScreen法; 蛋白アレイ; 新規自己抗体; AlphaScreen

Anti-cyclic citrullinated peptide (CCP) antibody is a specific autoantibody against rheumatoid arthritis (RA). However, it becomes positive in only about 50% of early RA patients, and sometimes it is difficult to correctly diagnose RA in the early phase. Therefore, the diagnostic biomarkers for anti-CCP antibody-negative RA have been required. We screened the anti-CCP antibody-negative RA sera for novel anti-citrullinated protein antibodies (ACPA) using AlphaScreen method. We found 14 novel anti-citrullinated protein antibodies from over 2,000 citrullinated proteins, out of which 2 were assured by Western blotting and ELISA.

抗杀菌柠檬酸肽（CCP）抗体是针对类风湿关节炎（RA）的特定自身抗体。但是，在早期RA患者中，它仅在约50％的早期患者中变得阳性，有时很难在早期阶段正确诊断RA。因此，需要用于抗CCP抗体阴性RA的诊断生物标志物。我们使用Alphascreen方法筛选了用于新型抗硝化蛋白抗体（ACPA）的抗CCP抗体阴离子Sera。我们发现了来自2,000多种瓜氨酸蛋白的14种新型抗硝化蛋白抗体，其中2种通过Western blotting和Elisa确保了2种。

项目成果

Recent advances on the genetics of rheumatoid arthritis: current topics and the future.

类风湿关节炎遗传学的最新进展：当前主题和未来。

• 发表时间: 2012
• 期刊: Inflamm Regen
• 影响因子: 8.1
• 作者: Ohmura K;Terao C;Mimori T.
• 通讯作者: Mimori T.

抗CCP抗体陰性関節リウマチに関連する遺伝因子

抗CCP抗体阴性类风湿性关节炎的相关遗传因素

• 发表时间: 2012
• 作者: Terao C;Ohmura K;Mimori T;et al.;鈴木和男;大村浩一郎
• 通讯作者: 大村浩一郎

PLD4 as a Novel Susceptibility Gene for Systemic Sclerosis in a Japanese Population

• DOI: 10.1002/art.37777
• 发表时间: 2013-02-01
• 期刊: ARTHRITIS AND RHEUMATISM
• 作者: Terao, Chikashi;Ohmura, Koichiro;Mimori, Tsuneyo
• 通讯作者: Mimori, Tsuneyo

Only rheumatoid factor-positive subset of anti-citrullinated peptide/protein antibody-negative rheumatoid arthritis seroconverts to anti-citrullinated peptide/protein antibody positive.

只有类风湿因子阳性的抗瓜氨酸肽/蛋白质抗体阴性类风湿性关节炎血清转变成抗瓜氨酸肽/蛋白质抗体阳性。

• 作者: Hiwa R;Ohmura K;Mimori T;et al
• 通讯作者: et al

Meta-analysis identifies nine new loci associated with rheumatoid arthritis in the Japanese population

• DOI: 10.1038/ng.2231
• 发表时间: 2012-05-01
• 期刊: NATURE GENETICS
• 影响因子: 30.8
• 作者: Okada, Yukinori;Terao, Chikashi;Yamamoto, Kazuhiko
• 通讯作者: Yamamoto, Kazuhiko