Screening of novel anti-citrullinated protein antibodies for rheumatoid arrhtiris by protein array

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Anti-cyclic citrullinated peptide (CCP) antibody is a specific autoantibody against rheumatoid arthritis (RA). However, it becomes positive in only about 50% of early RA patients, and sometimes it is difficult to correctly diagnose RA in the early phase. Therefore, the diagnostic biomarkers for anti-CCP antibody-negative RA have been required. We screened the anti-CCP antibody-negative RA sera for novel anti-citrullinated protein antibodies (ACPA) using AlphaScreen method. We found 14 novel anti-citrullinated protein antibodies from over 2,000 citrullinated proteins, out of which 2 were assured by Western blotting and ELISA.